Advanced glycation end products (AGEs) accumulate with age in human lens capsules. AGEs in lens capsules potentiate the transforming growth factor beta-2-mediated mesenchymal transition of lens epithelial cells, which suggests that they play a role in posterior capsule opacification after cataract surgery. We measured AGEs by liquid chromatography-mass spectrometry in capsulorhexis specimens obtained during cataract surgery from nondiabetic and diabetic patients with and without established retinopathy. Our data showed that the levels of most AGEs (12 out of 13 measured) were unaltered in diabetic patients and diabetic patients with retinopathy compared to nondiabetic patients. There was one exception: glucosepane, which was significantly higher in diabetic patients, both with (6.85 pmol/μmol OH-proline) and without retinopathy (8.32 pmol/μmol OH-proline), than in nondiabetic patients (4.01 pmol/μmol OH-proline). Our study provides an explanation for the similar incidence of posterior capsule opacification between nondiabetic and diabetic cataract patients observed in several studies.

高级糖基化终产物 (AGEs) 随着年龄的增长在人类晶状体囊中积累。晶状体囊中的 AGE 增强了转化生长因子 β-2 介导的晶状体上皮细胞间质转化，这表明它们在白内障手术后的后囊膜混浊中起作用。我们通过液相色谱-质谱法测量了在白内障手术期间从有和没有确定视网膜病变的非糖尿病和糖尿病患者获得的撕囊标本中的 AGEs。我们的数据显示，与非糖尿病患者相比，糖尿病患者和患有视网膜病变的糖尿病患者的大多数 AGEs 水平（测量的 13 个中的 12 个）没有改变。有一个例外：葡萄糖窗格，在糖尿病患者中显着更高，无论是有（6.85 pmol/μmol OH-脯氨酸）还是没有视网膜病变（8. 32 pmol/μmol OH-脯氨酸），高于非糖尿病患者（4.01 pmol/μmol OH-脯氨酸）。我们的研究为在几项研究中观察到的非糖尿病和糖尿病白内障患者后囊混浊的相似发生率提供了解释。