Sperm motility is a key event in fertilization, which is regulated by different factors. Among the factors that most affect sperm motility in fish are the external concentrations of Ca²⁺, the influence of which is highly regulated by various plasma membrane Ca²⁺-binding proteins. Concentrations of this cation have also been shown to be one of the most important factors affecting motility in cold-stored sperm cells. Taking into account the aforementioned aspects, we carried out a differential proteomic study on Salmo salar sperm stored at 4 °C with the aim of evaluating the protein profile on day 0 and day 14. The results of our study showed that 401 proteins were significantly downregulated (p < 0.05) on day 14, where four of them are key in the sperm motility of Salmo salar. The results of this study will allow a better understanding of the sperm activation mechanisms of Salmo salar, which will be of great importance in the design of future cold storage strategies for sperm preservation.

精子活力是受精过程中的一个关键事件，受不同因素的调节。影响鱼精子活力的最大因素是外部Ca ²⁺浓度，其影响受到各种质膜Ca ²⁺结合蛋白的高度调节。这种阳离子的浓度也已被证明是影响冷藏精子细胞运动的最重要因素之一。考虑到上述方面，我们对储存在 4°C 的鲑鱼精子进行了差异蛋白质组学研究，目的是评估第 0 天和第 14 天的蛋白质谱。我们的研究结果表明，401 种蛋白质显着下调( p <0.05) 在第 14 天，其中四个是Salmo salar精子活力的关键。本研究的结果将有助于更好地了解鲑鱼的精子激活机制，这对未来精子保存冷藏策略的设计具有重要意义。