The DMB2 gene is widely expressed at high levels in avian. This gene plays an important role in humoral immunity. The aim of this study was to investigate the effects of 361 G > C Single nucleotide polymorphism (SNP) on DMB2 protein structure and gene expression to determine how the 361 G > C SNP affects humoral immune response in Japanese quails. 0.2 mL of 5% sheep red blood cell (SRBC) was injected into breast muscle of 130 Japanese quails on 28 days. After DNA extraction, PCR was carried out to amplify a 333-base pair DNA fragment from the exon 2 of DMB2 gene. The pattern of all samples was determined through RFLP technique. PCR-RFLP results identified two alleles segregating (C, G) as three genotypes (CC, CG and GG) in Japanese Quails. The antibody response to SRBC with CC genotype was significantly higher than the CG and GG genotypes (P < 0.01). In silico analysis showed that the 361 G > C SNP has no effect on the physicochemical properties and 3D structure. The results of RT-qPCR indicated that the effect of genotype on gene expression is significant, so that the expression of CC genotype is more than CG and GG genotype. It can be inferred that the 361 G > C SNP in the exon 2 of MHC-DMB2 gene is not desirable. This mutation decreases humoral immune response by reducing DMB2 gene expression.

DMB2 基因在禽类中以高水平广泛表达。该基因在体液免疫中起重要作用。本研究的目的是研究 361 G > C 单核苷酸多态性 (SNP) 对 DMB2 蛋白结构和基因表达的影响，以确定 361 G > C SNP 如何影响日本鹌鹑的体液免疫反应。28天，130只日本鹌鹑胸肌内注射0.2 mL 5%绵羊红细胞（SRBC）。DNA提取后，进行PCR以从DMB2基因的外显子2扩增333个碱基对的DNA片段。所有样品的图案都是通过RFLP技术确定的。PCR-RFLP 结果将日本鹌鹑中的两个等位基因（C、G）鉴定为三种基因型（CC、CG 和 GG）。CC基因型对SRBC的抗体反应显着高于CG和GG基因型（P < 0.01）。计算机分析表明 361 G > C SNP 对物理化学性质和 3D 结构没有影响。RT-qPCR结果表明基因型对基因表达的影响显着，CC基因型的表达高于CG和GG基因型。可以推断，MHC-DMB2基因外显子2中的361 G > C SNP是不可取的。这种突变通过降低 DMB2 基因表达来降低体液免疫反应。因此CC基因型的表达量大于CG和GG基因型。可以推断，MHC-DMB2基因外显子2中的361 G > C SNP是不可取的。这种突变通过降低 DMB2 基因表达来降低体液免疫反应。因此CC基因型的表达量大于CG和GG基因型。可以推断，MHC-DMB2基因外显子2中的361 G > C SNP是不可取的。这种突变通过降低 DMB2 基因表达来降低体液免疫反应。