Abstract

The purpose of the present study was to examine the protective action and mechanisms of quercetin on the blood-brain barrier (BBB) in rats subjected to transient middle cerebral artery occlusion (tMCAO) and reperfusion. Quercetin (10, 30, 50 mg/kg) was intraperitoneally administered at the onset of reperfusion. The results showed that quercetin significantly reduced cerebral infarct volume, neurological deficit, BBB permeability and ROS generation via Sirt1/Nrf2/HO-1 signaling pathway. Moreover, EX527, a selective inhibitor of Sirt1, reversed these neuroprotective effects. Our findings indicate that quercetin has neuroprotective effects against cerebral ischemia-reperfusion injury by protecting BBB through Sirt1 signaling pathway in MCAO rats.

摘要

本研究的目的是研究槲皮素对短暂大脑中动脉闭塞 (tMCAO) 和再灌注大鼠血脑屏障 (BBB) 的保护作用和机制。在再灌注开始时腹膜内施用槲皮素（10、30、50 mg/kg）。结果表明，槲皮素通过Sirt1/Nrf2/HO-1信号通路显着降低脑梗死体积、神经功能缺损、血脑屏障通透性和活性氧生成。此外，Sirt1 的选择性抑制剂 EX527 可逆转这些神经保护作用。我们的研究结果表明，槲皮素通过 Sirt1 信号通路保护 MCAO 大鼠的 BBB，对脑缺血再灌注损伤具有神经保护作用。