Tomato is the world’s second largest cultivated vegetable crop. Tomato spotted wilt virus (TSWV) and fusarium wilt (FW) are the two major biotic stresses in India limiting tomato production. Identification and utilization of resistant lines to realize the full genetic potential of varieties for yield gain is an eco-friendly approach. The present research work involved genetic diversity study of 48 genotypes, augmented from different exotic, and indigenous sources belonging to three species using SSR markers. A total of 195 alleles were generated by employing 84 polymorphic markers. The PIC value was ranged from 0.12 to 0.93. Two sub-populations (K = 2) were revealed by model based structure analysis. The cluster analysis using the UPGMA method classified the genotypes into 6 clusters. Pusa Ruby, EC-310310 and EC-620452 were found to be highly diverse. Molecular characterization of 48 genotypes with SSR markers divulged seven genotypes with Sw-5 gene and nine genotypes with I-2 gene showing resistance to TSWV and FW, respectively and further, on artificial screening, they were found to be phenotypically resistant. Out of 195 alleles generated from 84 polymorphic SSR markers, 43 alleles from 26 SSR markers were identified with positive average allele effect distributed across nine chromosomes and positive average allele effect was identified for the average weight of the fruit, the number of fruits formed per plant, and fusarium wilt PDI score. Fruit weight and fruit yield per plant registered a significant and positive correlations. The identified genotypes with varied backgrounds and performances will be very useful as diversified sources in resistant breeding programs of tomato.

番茄是世界第二大栽培蔬菜作物。番茄斑萎病毒 (TSWV) 和枯萎病 (FW) 是印度限制番茄生产的两种主要生物胁迫。鉴定和利用抗性品系以实现品种的全部遗传潜力以提高产量是一种生态友好的方法。目前的研究工作涉及使用 SSR 标记从属于三个物种的不同外来和本土来源的 48 种基因型的遗传多样性研究。通过使用 84 个多态性标记，总共产生了 195 个等位基因。PIC 值范围为 0.12 至 0.93。通过基于模型的结构分析揭示了两个亚群 (K = 2)。使用 UPGMA 方法的聚类分析将基因型分为 6 个聚类。菩萨红宝石，EC-310310 和 EC-620452 被发现高度多样化。用 SSR 标记对 48 种基因型进行分子表征揭示了 7 种基因型Sw-5基因和9个具有I-2基因的基因型分别表现出对TSWV和FW的抗性，进一步，在人工筛选中，发现它们具有表型抗性。在从 84 个多态性 SSR 标记产生的 195 个等位基因中，从 26 个 SSR 标记中鉴定出 43 个等位基因，其平均等位基因效应分布在 9 条染色体上，平均等位基因效应为平均果实重量，每株形成的果实数量和枯萎病 PDI 评分。单株单果重和单果产量呈显着正相关。所鉴定的具有不同背景和表现的基因型将作为番茄抗性育种计划的多样化来源非常有用。