Abstract

In previous study, we identified a novel protein elicitor AMEP412 from Bacillus subtilis BU412, which could trigger plant defense response and induce systemic acquired resistance against diseases. In this paper, the production of the new elicitor was optimised. First, optimal carbon source and nitrogen source were determined as glucose and yeast extract by single factor test. Then, effects of seven different factors on AMEP412 production was studied by Plackett-Burman design. Significant factors (yeast extract, culture temperature and culture time) were selected for further optimisation using central composite design by response surface methodology. The optimum conditions for AMEP412 production were yeast extract 21.72 g/L, culture temperature 30.84 °C and culture time 31.84 h. Under these conditions, the yield of AMEP412 was enhanced from 0.32 to 2.39 mg/mL (7.47-fold), which was quite close to the predicted one. This result significantly raised the yield of AMEP412, which laid solid foundation for its application as a biocontrol agent.

摘要

在之前的研究中，我们从枯草芽孢杆菌中鉴定了一种新型蛋白诱导剂 AMEP412BU412，可触发植物防御反应并诱导系统性获得性抗病性。在本文中，优化了新诱导物的生产。首先，通过单因素试验确定最佳碳源和氮源为葡萄糖和酵母提取物。然后，通过 Plackett-Burman 设计研究了七种不同因素对 AMEP412 产生的影响。选择重要因素（酵母提取物、培养温度和培养时间）以通过响应面方法使用中心复合设计进行进一步优化。AMEP412生产的最佳条件是酵母提取物21.72 g/L，培养温度30.84 °C，培养时间31.84 h。在这些条件下，AMEP412 的产量从 0.32 增加到 2.39 mg/mL（7.47 倍），与预测值非常接近。