The purpose of this study was to evaluate the effect of time in culture on fatty acid composition of copepods Tisbe sp. and Apocyclops sp. For experiments, Tisbe and Apocyclops copepodites were separated and placed in 4-L containers at 5 ind/mL. The fatty acids were evaluated at three different periods (hours) of culture (treatments): T1 = 6 hr, T2 = 12 hr, and T3 = 168 hr. Culture conditions were 25°C, salinity 35 ppt, illuminance 2,500 lx, with gentle aeration. The live food was Isochrysis galbana at 75 × 10³ cells/mL. A total of 14 fatty acids were detected for both copepods. Although, there were absent two fatty acids for Apocyclops sp. (C18:3n-6 and C18:1n-7). The major percentages of fatty acids were more than 50% of polyunsaturated fatty acids. The essential fatty acids were detected such as C22:6n-3 (docosahexaenoic acid [DHA]), C20:5n-3 (eicosapentaenoic acid [EPA]), and C20:4n-6 (arachidonic acid) for both species. Tisbe sp. was the species which shows the highest quantity of total lipids compared to Apocyclops sp. for the three treatments. Besides, in T3 both species had greater quantities of total lipids in respect to the first treatment. The ratio of DHA/EPA was optimal for culture of fish marine larvae because the proportion is higher than 2:1.

中文翻译：

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培养时间对桡足类 Tisbe sp. 脂肪酸组成的影响 和 Apocyclops sp。

本研究的目的是评估培养时间对桡足类Tisbe sp脂肪酸组成的影响。和Apocyclops sp。对于实验，将 Tisbe和Apocyclops桡足动物分离并以 5 ind/mL 的浓度放置在 4-L 容器中。在培养（处理）的三个不同时期（小时）评估脂肪酸：T1 = 6 小时、T2 = 12 小时和 T3 = 168 小时。培养条件为 25°C，盐度 35 ppt，照度 2,500 lx，温和通气。活的食物是75 × 10 ^{3 个}细胞/mL的等鞭金藻。两种桡足类共检测到 14 种脂肪酸。虽然， Apocyclops sp没有两种脂肪酸. （C18:3n-6 和 C18:1n-7）。脂肪酸的主要百分比是多不饱和脂肪酸的50%以上。对这两个物种都检测到了必需脂肪酸，例如 C22:6n-3（二十二碳六烯酸 [DHA]）、C20:5n-3（二十碳五烯酸 [EPA]）和 C20:4n-6（花生四烯酸）。Tisbe sp . 是与Apocyclops sp相比，总脂质含量最高的物种。对于三个治疗。此外，在 T3 中，与第一次处理相比，两个物种的总脂质含量更高。DHA/EPA 的比例高于 2:1，最适合养殖鱼类海生幼体。