We studied immunolocalization of CD29, CD44, osteocalcin, and TGF-β1 in the bone tissue of the mandible of miniature pigs with extra-bone fixation of a free gingival graft. Three months after surgery, neoosteogenesis foci with high expression of the studied markers were found in the contact area of the free gingival graft with the alveolar bone. The markers were localized in the layer of external circumferential lamellae, on the surface of concentric lamellae of the growing osteons, and in the connective tissue of the Haversian canals. TGF-β1-immunopositive cells predominated in the connective tissue of the Haversian and Volkmann canals and in the adventitia and inner lining of the vascular wall. The established morphochemical patterns of osteogenous cells indicate significant reparative capabilities of a free gingival graft and allows considering it as an effective osteoinductive factor.

我们研究了用游离牙龈移植物进行骨外固定的小型猪下颌骨组织中 CD29、CD44、骨钙素和 TGF-β1 的免疫定位。术后三个月，在游离牙龈移植物与牙槽骨的接触区域发现了具有高表达研究标记的新骨生成病灶。标记物定位于外周板层、生长骨的同心板层表面以及哈弗氏管的结缔组织中。TGF-β1 免疫阳性细胞主要存在于哈弗斯管和沃尔克曼管的结缔组织以及血管壁的外膜和内层中。