The objective was to evaluate meat quality of Rubia Gallega x Nelore (RGN) crossbred compared to Nelore (N) cattle, using mRNA analysis of calpain 1 and 2, calpastatin, myostatin, and TGFβ; and protein abundance profile post mortem based on aged beef of the different genetic groups. Sixteen N and 16 RGN were finished in feedlot by 120 days and harvested. RGN beef presented greater tenderness. Transcripts analysis showed increased levels of TGFβ and MSTN in RGN, indicating differences in muscle fiber formation. However, no difference was observed in mRNA for CAPN1, CAPN2 and CAST. Protein analysis demonstrated increased troponin-T degradation in RGN. Interaction for HSP 27 degradation was observed, indicating the degradation of this protein is faster and greater in RGN than N. No difference in myostatin protein abundance was found. In general, RGN cattle improves beef quality and these differences may be explained by TGFβ and MSTN gene expression, troponin-T degradation and HSP abundance.

目的是使用钙蛋白酶 1 和 2、钙蛋白酶抑制素、肌生长抑制素和TGFβ 的mRNA 分析来评估 Rubia Gallega x Nelore (RGN) 杂交牛与 Nelore (N) 牛相比的肉质；以及基于不同基因组的陈年牛肉的死后蛋白质丰度曲线。到 120 天时，16 只 N 和 16 只 RGN 在饲养场中完成并收获。RGN 牛肉呈现出更大的嫩度。转录本分析显示RGN中TGFβ和MSTN水平增加，表明肌肉纤维形成存在差异。然而，在CAPN1、CAPN2和CAST 的mRNA 中没有观察到差异. 蛋白质分析表明 RGN 中肌钙蛋白-T 降解增加。观察到 HSP 27 降解的相互作用，表明该蛋白质在 RGN 中的降解比 N 更快、更大。未发现肌肉生长抑制素蛋白丰度的差异。一般而言，RGN 牛提高了牛肉质量，这些差异可以通过TGFβ和MSTN基因表达、肌钙蛋白-T 降解和 HSP 丰度来解释。