Background Alternaria toxins are ubiquitous contaminants in highly consumed food products. Therefore, they are candidates to be regulated by EU legislation. In this context, the availability of reliable analytical methods is a keystone both for protecting the health of citizens and smooth functioning of the European market. Objective This paper describes an advanced LC-MS/MS method based on isotope dilution quantification suitable for the determination of altenuene, alternariol, alternariol monomethyl ether, tenuazonic acid, and tentoxin in tomato puree, wheat, and sunflower seeds. Methods The method has been validated in an interlaboratory study that included the analysis of both spiked and naturally contaminated food commodities. Twenty-three participants contributed with analytical data. Results The average recoveries and relative standard deviations for repeatability and reproducibility obtained across the tested matrixes were: 97, 8.0, and 23%, for altenuene, respectively; 95, 9.2, and 17% for alternariol, respectively; 98, 6.4, and 13% for alternariol monomethyl ether, respectively; 97, 4.2, and 9.3% for tenuazonic acid, respectively; and 102, 5.6, and 15% for tentoxin, respectively. The method enabled the determination of all tested Alternaria toxins close to or below 1 µg/kg. Conclusion Overall, the method showed a satisfactory trueness and precision, complying with the requirements for the monitoring of mycotoxins in food in the EU. It is currently under evaluation by the European Committee for Standardization for adoption as a standard method. Highlights Isotope dilution mass spectrometry method for the determination of Alternaria toxins in food.

中文翻译：

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通过 SPE 和 LC-MS/MS 测定番茄、小麦和葵花籽中的链格孢毒素——通过合作试验验证方法

背景 链格孢毒素是高消费食品中普遍存在的污染物。因此，它们是受欧盟立法监管的候选者。在这种情况下，可靠的分析方法的可用性是保护公民健康和欧洲市场顺利运作的基石。目的 本文介绍了一种先进的基于同位素稀释定量的 LC-MS/MS 方法，适用于测定番茄泥、小麦和葵花籽中的艾烯、交替烯醇、交替烯醇单甲醚、tenuazonic acid 和tentoxin。方法 该方法已在一项实验室间研究中得到验证，该研究包括对加标和自然污染食品的分析。23 名参与者提供了分析数据。结果 在测试的基质中获得的重复性和重现性的平均回收率和相对标准偏差分别为： 97%、8.0% 和 23%，对于 altenuene；交替使用 95%、9.2% 和 17%；98%、6.4% 和 13% 的交替萘酚单甲醚，分别为；分别为 97、4.2 和 9.3% 的替萘唑酸；和 102, 5.6, 和 15% 分别为十毒素。该方法能够测定接近或低于 1 µg/kg 的所有受试链格孢毒素。结论 总体而言，该方法的正确性和精密度较好，符合欧盟食品中霉菌毒素的监测要求。目前，欧洲标准化委员会正在对其进行评估，以将其作为标准方法采用。