Immune cells are generated from hematopoietic stem cells (HSCs) in the bone marrow (BM). Immune stimulation can rapidly activate HSCs out of their quiescent state to accelerate the generation of immune cells. HSCs’ activation follows various viral or bacterial stimuli, and we sought to investigate the hypersensitivity immune response. Surprisingly, the Ova-induced hypersensitivity peritonitis model finds no significant changes in BM HSCs. HSC markers cKIT, SCA1, CD48, CD150, and the Fgd5-mCherry reporter showed no significant difference from control. Functionally, hypersensitivity did not alter HSCs' potency, as assayed by transplantation. We further characterized the possible impact of hypersensitivity using RNA-sequencing of HSCs, finding minor changes at the transcriptome level. Moreover, hypersensitivity induced no significant change in the proliferative state of HSCs. Therefore, this study suggests that, in contrast to other immune stimuli, hypersensitivity has no impact on HSCs.

免疫细胞由骨髓 (BM) 中的造血干细胞 (HSC) 产生。免疫刺激可以迅速激活 HSCs 使其脱离静止状态，从而加速免疫细胞的产生。HSCs 的激活遵循各种病毒或细菌刺激，我们试图研究超敏性免疫反应。令人惊讶的是，Ova 诱导的超敏性腹膜炎模型发现 BM HSCs 没有显着变化。HSC 标记 cKIT、SCA1、CD48、CD150 和 Fgd5-mCherry 报告基因与对照没有显着差异。在功能上，超敏反应并没有改变 HSCs 的效力，正如移植所分析的那样。我们使用 HSC 的 RNA 测序进一步描述了超敏反应的可能影响，发现转录组水平的微小变化。而且，超敏反应不会引起 HSCs 增殖状态的显着变化。因此，本研究表明，与其他免疫刺激相比，超敏反应对 HSC 没有影响。