Rapid bioassay method for plutonium in urine samples are employed to assess the possible internal dose resulting from exposure to plutonium in the event of a radiological and nuclear emergency. In this work, a rapid method for the determination of plutonium isotopes (²³⁹Pu and ²⁴⁰Pu) in large volume of urine samples was developed using tandem quadrupole ICP-MS combined with a high-efficiency sample introduction system (APEX-Ω). The mixture gases of NH₃ and He was employed to effectively eliminate the tailing of ²³⁸U and the uranium hydride ions (²³⁸U¹H⁺and ²³⁸U¹H₂⁺) and significantly improve the measurement sensitivity of plutonium. The method was validated by analyzing urine samples spiked with known amounts of ²³⁹Pu and ²⁴⁰Pu ranging from 1 to 60 fg. The detection limits for the method were estimated to be 0.83 fg for ²³⁹Pu, 0.14 fg for ²⁴⁰Pu in 1 L of urine.

尿样中钚的快速生物测定方法用于评估在放射性和核紧急情况下接触钚可能导致的内部剂量。在这项工作中，使用串联四极杆 ICP-MS 结合高效进样系统 (APEX-Ω) 开发了一种快速测定大量尿液样品中钚同位素（²³⁹ Pu 和²⁴⁰ Pu）的方法。采用NH ₃和He混合气体有效去除²³⁸ U 和铀氢阴离子（²³⁸ U ¹ H ⁺和²³⁸ U ¹ H ₂ ⁺) 并显着提高钚的测量灵敏度。该方法通过分析加标了已知量的²³⁹ Pu 和²⁴⁰ Pu，范围从 1 到 60 fg 的尿液样品得到验证。该方法的检测限估计为1 L 尿液中²³⁹ Pu的检测限为 0.83 fg，²⁴⁰ Pu的检测限为0.14 fg 。