Abstract

On account of unique mechanical property and inertia, parylene-C has become a promising material for microdevices especially in three-dimensional microstructures loaded with cells. However, parylene-C is not favorable for cell adhesion, and a routine procedure is to modify it with a new adhesive layer. Herein, the parylene-C substrates with or without collagen Ӏ (Col-I) coating were adopted to estimate the influence of micro-environment change on cell attachment and spreading. After modification with Col-I, cauliflower-like particles presented on the substrate surface. Contact angle was significantly decreased after Col-I modification, which suggested the surface hydrophilicity was enhanced. Furthermore, cells cultured on parylene-C surface with Col-I treatment showed increased proliferation rate and spreading areas. In order to test the adhesion strength, a series of fixed size parylene-C microplates was fabricated, and cell suspension concentration was adjusted to culture a single cell on one microplate. The microplate was folded by the autogenous shrinkage force of cell. The folding angles of parylene-C microplates with Col-I treatment exhibited higher folding angle (112.6 ± 15.6°) than untreated samples (46.7 ± 5.9°). The work proved the existence of Col-I layer was particularly important, especially in analysis of cells mechanics using parylene-C microplate as a substrate.

摘要

由于独特的机械性能和惯性，聚对二甲苯-C已成为一种很有前途的微型器件材料，特别是在装有细胞的三维微结构中。然而，parylene-C 不利于细胞粘附，常规程序是用新的粘附层对其进行修改。在此，采用带有或不带有胶原蛋白Ӏ (Col-I) 涂层的聚对二甲苯-C 底物来估计微环境变化对细胞附着和扩散的影响。用 Col-I 修饰后，花椰菜状颗粒出现在基材表面。Col-I改性后接触角显着降低，表明表面亲水性增强。此外，用 Col-I 处理在聚对二甲苯-C 表面上培养的细胞显示出增加的增殖率和扩散区域。为了测试粘附强度，制作了一系列固定尺寸的聚对二甲苯-C微孔板，并调整细胞悬液浓度以在一个微孔板上培养单个细胞。微孔板被细胞的自收缩力折叠。用 Col-I 处理的聚对二甲苯-C 微孔板的折叠角 (112.6 ± 15.6°) 比未处理的样品 (46.7 ± 5.9°) 显示出更高的折叠角。这项工作证明了 Col-I 层的存在特别重要，尤其是在使用聚对二甲苯-C 微孔板作为基材的细胞力学分析中。用 Col-I 处理的聚对二甲苯-C 微孔板的折叠角 (112.6 ± 15.6°) 比未处理的样品 (46.7 ± 5.9°) 显示出更高的折叠角。这项工作证明了 Col-I 层的存在特别重要，尤其是在使用聚对二甲苯-C 微孔板作为基材的细胞力学分析中。用 Col-I 处理的聚对二甲苯-C 微孔板的折叠角 (112.6 ± 15.6°) 比未处理的样品 (46.7 ± 5.9°) 显示出更高的折叠角。这项工作证明了 Col-I 层的存在特别重要，尤其是在使用聚对二甲苯-C 微孔板作为基材的细胞力学分析中。