A pH indicator, bromothymol blue, which changes color from yellow to blue in alkaline conditions, was used to directly detect, with the naked eye, clones expressing active transketolase from Geobacillus stearothermophilus (wild type or variants) in combination with donor (α-ketoacids) and acceptor substrates (aldehydes), obviating enzyme extraction.

中文翻译：

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用于底物分析和转酮酶定向进化的高通量固相测定

pH 指示剂溴百里酚蓝（在碱性条件下颜色从黄色变为蓝色）用于直接检测表达来自嗜热脂肪地芽孢杆菌（野生型或变体）的活性转酮醇酶的克隆与供体（α-酮酸）和受体底物（醛），避免酶提取。