Journal of Essential Oil Bearing Plants ( IF 2.4 ) Pub Date : 2021-07-20 , DOI: 10.1080/0972060x.2021.1944326 Dridi Imen 1 , Haouel-Hamdi Soumaya 2 , Cheraief Imed 3 , Mediouni Ben Jemâa Jouda 2 , Landoulsi Ahmed 1 , Chaouch Rym 1, 4
Abstract
In this study, the chemical composition, antioxidant, antimicrobial, and insecticidal activities of essential oil (EO) from Lavandula dentata (L. dentata) flowering tops collected from northern Tunisia were assessed. Forty-three compounds were identified. β-eudesmol (21.178 %), myrtenol (13.023 %), and sabinol (11.021 %) were the main compounds. The antibacterial potential was evaluated against three Gram-positive bacteria and three Gram-negative bacteria. The diameter of inhibition varied between 20.83±0.47 mm and 24.16±0.47 mm. The largest one was found in Salmonella typhimurium, however, the smallest one was found in Bacillus cereus. The minimal inhibition concentration (MIC) varied between 0.1 and 0.6 μg/ml. Furthermore, the antifungal potential was carried out. The mycelial growth of all the treated fungi was significantly different from the control ones (p<0.05), except in the case of P. notatum when treated with a dose of 1.25 mg/ml. Moreover, L. dentata EO was screened for its antioxidant activity using three methods. IC50 values were 113.29± 0.012 mg/ml, 53.029± 0.02 mg/ml, 43.20± 0.004 mg/ml, respectively for the DPPH test, the ABTS test and the reducing power test. The insecticidal activity was tested against adults of two major insect pests namely Rhyzopertha dominica and Sitophilus oryzae using contact, fumigant toxicity, and repellent bioassays. Results revealed that the L. dentata EO exhibited strong fumigant, repellent, and contact properties. For all tests, R. dominica was more sensitive compared to S. oryzae. This study revealed that EO from L. dentata flowering tops could be potentially valorized as a new antibacterial, antifungal, and bioinsecticide product.
中文翻译:
来自 Lavandula dentata (L) 花顶的精油:化学成分、抗菌、抗氧化和杀虫活性
摘要
在这项研究中,评估了从突尼斯北部采集的Lavandula dentata ( L. dentata ) 开花顶部精油 (EO) 的化学成分、抗氧化、抗菌和杀虫活性。鉴定出 43 种化合物。β-eudesmol (21.178 %)、桃金娘醇 (13.023 %) 和 sabinol (11.021 %) 是主要化合物。针对三种革兰氏阳性菌和三种革兰氏阴性菌评估了抗菌潜力。抑制直径在 20.83±0.47 毫米和 24.16±0.47 毫米之间变化。最大的一种是在鼠伤寒沙门氏菌中发现的,而最小的一种是在蜡状芽孢杆菌中发现的. 最小抑制浓度 (MIC) 在 0.1 和 0.6 μg/ml 之间变化。此外,进行了抗真菌潜力。所有处理过的真菌的菌丝体生长都与对照显着不同(p <0.05),除了用 1.25 mg/ml 剂量处理的P. notatum之外。此外,使用三种方法筛选了L. dentata EO 的抗氧化活性。对于DPPH测试、ABTS测试和还原能力测试,IC 50值分别为113.29±0.012mg/ml、53.029±0.02mg/ml、43.20±0.004mg/ml。对两种主要害虫,即多米尼卡丝虫和米粒线虫的成虫进行了杀虫活性测试使用接触、熏蒸剂毒性和驱避生物测定法。结果表明,L. dentata EO 表现出很强的熏蒸、驱避和接触特性。对于所有测试,R. dominica比S. oryzae更敏感。这项研究表明,来自L. dentata开花顶部的EO可能被视为一种新的抗菌、抗真菌和生物杀虫剂产品。