Mesenchymal stem cells (MSCs) have received considerable attention as therapeutic cells for regenerative medicine and tissue engineering, because of their ability to replace damaged cells or regenerate surrounding cells. There are many technical difficulties in the mass production of high-quality stem cells because the stem cells must maintain an efficient proliferative cell state during in vitro culture. The results of this study show that plasma surface-modification enhanced significantly the culture of adipose-derived mesenchymal stem cells (ASCs) on the polystyrene (PS) Petri dishes. Ar, O₂, pyrrole, and 4,7,10-trioxa-1,13-tridecanediamine (TTDDA) were used as the gas and/or precursors for plasma modification. Specifically, surfaces of PS Petri dishes, coated with plasma polymerized pyrrole (ppPy) and plasma polymerized TTDDA (ppTTDDA) were found to contain amine and carboxyl functional groups, respectively. Ar and O₂ plasma-treated PS Petri dishes have similar culture abilities (±1.2 times) to commercially available tissue culture polystyrene (TCPS) dishes, and PS Petri dishes coated with ppPy and ppTTDDA have significantly enhanced culture abilities (2.4 times) at 96 hr compared with TCPS dishes. Western blotting was performed using antibodies against stem cell marker proteins to confirm the stemness properties of stem cells, in the sense that the expressions of the antibody proteins such as CD44, CD73, and CD105 in plasma modified samples were similar to or higher than those in TCPS dishes.

中文翻译：

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在由等离子体增强化学气相沉积系统制造的表面改性聚苯乙烯培养皿上增强脂肪来源间充质干细胞的培养

间充质干细胞 (MSCs) 作为再生医学和组织工程的治疗细胞受到了相当多的关注，因为它们具有替换受损细胞或再生周围细胞​​的能力。由于干细胞在体外培养过程中必须保持高效的增殖细胞状态，因此大规模生产高质量干细胞存在许多技术难题。这项研究的结果表明，血浆表面修饰显着增强了聚苯乙烯 (PS) 培养皿上脂肪来源的间充质干细胞 (ASC) 的培养。氩，氧₂、吡咯和 4,7,10-trioxa-1,13-十三烷二胺 (TTDDA) 用作等离子体改性的气体和/或前体。具体来说，发现涂有等离子聚合吡咯 (ppPy) 和等离子聚合 TTDDA (ppTTDDA) 的 PS 培养皿表面分别含有胺和羧基官能团。Ar和O ₂经等离子体处理的 PS 培养皿与市售的组织培养聚苯乙烯 (TCPS) 培养皿具有相似的培养能力（±1.2 倍），与 TCPS 相比，涂有 ppPy 和 ppTTDDA 的 PS 培养皿在 96 小时时具有显着增强的培养能力（2.4 倍）菜。使用针对干细胞标记蛋白的抗体进行蛋白质印迹以确认干细胞的干细胞特性，即血浆修饰样品中 CD44、CD73 和 CD105 等抗体蛋白的表达与血浆修饰样品中的相似或更高。 TCPS 菜肴。