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Characterization of lipoprotein lipase storage vesicles in 3T3-L1 adipocytes.
Journal of Cell Science ( IF 4 ) Pub Date : 2021-08-12 , DOI: 10.1242/jcs.258734
Benjamin S Roberts 1 , Chelsea Q Yang 1 , Saskia B Neher 1
Affiliation  

Lipoprotein lipase (LPL) is a secreted triglyceride lipase involved in the clearance of very-low-density lipoproteins and chylomicrons from circulation. LPL is expressed primarily in adipose and muscle tissues and transported to the capillary lumen. LPL secretion is regulated by insulin in adipose tissue; however, few studies have examined the regulatory and trafficking steps involved in secretion. Here, we describe the intracellular localization and insulin-dependent trafficking of LPL in 3T3-L1 adipocytes. We compared LPL trafficking to the better characterized trafficking pathways taken by leptin and GLUT4 (also known as SLC2A4). We show that the LPL trafficking pathway shares some characteristics of these other pathways, but that LPL subcellular localization and trafficking are distinct from those of GLUT4 and leptin. LPL secretion occurs slowly in response to insulin and rapidly in response to the Ca2+ ionophore ionomycin. This regulated trafficking is dependent on Golgi protein kinase D and the ADP-ribosylation factor GTPase ARF1. Together, these data give support to a new trafficking pathway for soluble cargo that is active in adipocytes.

中文翻译:

3T3-L1 脂肪细胞中脂蛋白脂肪酶储存囊泡的表征。

脂蛋白脂肪酶 (LPL) 是一种分泌型甘油三酯脂肪酶,参与循环中极低密度脂蛋白和乳糜微粒的清除。LPL 主要在脂肪和肌肉组织中表达并转运至毛细血管腔。LPL的分泌受脂肪组织中胰岛素的调节;然而,很少有研究考察分泌过程中涉及的调控和运输步骤。在这里,我们描述了 3T3-L1 脂肪细胞中 LPL 的细胞内定位和胰岛素依赖性运输。我们将 LPL 运输与瘦素和 GLUT4(也称为 SLC2A4)所采用的更具有特征的运输途径进行了比较。我们表明,LPL 运输途径与其他途径具有一些共同特征,但 LPL 亚细胞定位和运输与 GLUT4 和瘦素的亚细胞定位和运输不同。LPL 分泌对胰岛素反应缓慢,对 Ca2+ 离子载体离子霉素反应迅速。这种调节运输依赖于高尔基体蛋白激酶 D 和 ADP-核糖基化因子 GTPase ARF1。总之,这些数据支持了脂肪细胞中活跃的可溶性货物的新运输途径。
更新日期:2021-07-19
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