Intravital microscopy (IVM) has revolutionized our understanding of single-cell behavior in complex tissues by enabling real-time observation of molecular and cellular processes in their natural environment. In preclinical research, IVM has emerged as a standard tool for mechanistic studies of therapy response and the rational design of new treatment strategies. Technological developments keep expanding the imaging depth and quality that can be achieved in living tissue, and the maturation of imaging modalities such as fluorescence and phosphorescence lifetime imaging facilitates co-registration of individual cell dynamics with metabolic tissue states. Correlation of IVM with mesoscopic and macroscopic imaging modalities further promotes the translation of mechanistic insights gained by IVM into clinically relevant information. This review highlights some of the recent advances in IVM that have made the transition from experimental optical techniques to practical applications in basic and preclinical research.

中文翻译：

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活体显微镜用于临床前研究的最新进展。

活体显微镜 (IVM) 通过实时观察自然环境中的分子和细胞过程，彻底改变了我们对复杂组织中单细胞行为的理解。在临床前研究中，IVM 已成为治疗反应机制研究和合理设计新治疗策略的标准工具。技术发展不断扩大可在活组织中实现的成像深度和质量，荧光和磷光寿命成像等成像方式的成熟促进了个体细胞动力学与代谢组织状态的共同配准。IVM 与细观和宏观成像模式的相关性进一步促进了将 IVM 获得的机械见解转化为临床相关信息。