Abstract

Colletotrichum lentis is an important pathogen of lentils in Canada causing the disease anthracnose. Fungicide applications are an important tool for the control of this disease and, historically, QoI fungicidegdels have been used intensively. Reduced field efficacy of QoI fungicides on C. lentis has been reported recently and could be linked to the presence of QoI resistant isolates. In this study, the cytochrome b gene of C. lentis was sequenced, and the mutation G143A was identified as the causal resistance mechanism. Furthermore, a sensitive and reliable molecular biological assay was developed for the quantitative detection of the G143A mutation by pyrosequencing in field samples.

摘要

Colletotrichum lentis是加拿大重要的扁豆病原菌，可引起炭疽病。杀真菌剂的应用是控制这种疾病的重要工具，从历史上看，QoI 杀真菌剂已被广泛使用。最近报道了 QoI 杀菌剂对C. lentis的田间效力降低，这可能与 QoI 抗性分离株的存在有关。本研究对C. lentis的细胞色素b基因进行测序，确定突变G143A为致病耐药机制。此外，还开发了一种灵敏可靠的分子生物学检测方法，用于通过焦磷酸测序定量检测野外样品中的 G143A 突变。