Photodynamic Therapy (PDT) seems to be a promising method in the treatment of larynx tumor tissues. The aim of the present analysis was the study of photosensitizer penetration of larynx tissue associated with the application of PDT in vitro. This study is based on the use of photosensitive compounds Rose Bengal (RB) that selectively accumulate in larynx tissue. The selection of the study group of patients who will undergo surgery in accordance with medical principles was of key importance for the project. Histopathological examination of samples subjected to PDT revealed numerous changes in the morphology of the cancer cells and surrounding tissues. After PDT treatment, the number of tumor cells decreased compared with the cells number before PDT and the arrangement was relatively loose. After PDT with RB the nuclei morphology was incomplete and fragmented. The effects of the applied PDT of larynx in vitro were assessed under an optical microscope. The future directions in larynx tumor PDT with the use of upconversion nanoparticles (UPCNP) is also discussed.

中文翻译：

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玫瑰红和喉肿瘤光动力治疗的未来方向†

光动力疗法（PDT）似乎是治疗喉肿瘤组织的一种有前途的方法。本分析的目的是研究与体外 PDT 应用相关的喉组织的光敏剂渗透。这项研究基于使用选择性积聚在喉组织中的光敏化合物玫瑰红 (RB)。选择将按照医学原则接受手术的患者研究组对该项目至关重要。接受 PDT 的样本的组织病理学检查显示癌细胞和周围组织的形态发生了许多变化。PDT治疗后肿瘤细胞数较PDT前减少，排列较松散。用 RB 进行 PDT 后，细胞核形态不完整且碎片化。在光学显微镜下评估体外应用喉部PDT的效果。还讨论了使用上转换纳米粒子 (UPCNP) 进行喉肿瘤 PDT 的未来方向。