In this study, anthocyanins were extracted and purified from purple sweet potato anthocyanins (PSPA) and the alleviative effect of PSPA on doxorubicin (DOX)-induced cardiotoxicity was investigated. High Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS) results showed that 10 kinds of substances were identified in PSPA and the PSPA was mainly composed of cyanidin (62.9%) and peonidin (21.46%). In in vitro experiments, PSPA reduced the excessive release of inflammatory factors (NO and TNF-α) induced by DOX and decreased the secretion of trimethylamine oxide (TMAO), lactic dehydrogenase (LDH), and creatine kinase (CK) caused by myocardial injury. In in vivo experiments, PSPA inhibited the release of NO and MDA levels in heart tissue. Meanwhile, mice treated with PSPA decreased the levels of LDH, CK, TNF-α, and TMAO in serum and heart tissue when compared with the DOX group. In addition, the histopathological results of the heart tissue also showed a protective effect of PSPA on the pathological changes in heart. These results provide a reference for the application of PSPA as a functional food to intervene in DOX-induced cardiotoxicity.

中文翻译：

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紫甘薯花青素在体外和体内减轻阿霉素诱导的心脏毒性

在这项研究中，从紫甘薯花青素 (PSPA) 中提取和纯化了花青素，并研究了 PSPA 对阿霉素 (DOX) 诱导的心脏毒性的缓解作用。高效液相色谱-质谱(HPLC-MS)结果表明，在PSPA中鉴定出10种物质，其中PSPA主要由花青素(62.9%)和芍药苷(21.46%)组成。在体外实验中，PSPA减少了DOX诱导的炎症因子（NO和TNF-α）的过度释放，减少了心肌损伤引起的氧化三甲胺（TMAO）、乳酸脱氢酶（LDH）和肌酸激酶（CK）的分泌. 在体内实验中，PSPA 抑制了心脏组织中 NO 和 MDA 水平的释放。同时，用 PSPA 治疗的小鼠降低了 LDH、CK、TNF-α、与 DOX 组相比，血清和心脏组织中的 TMAO 和 TMAO。此外，心脏组织的组织病理学结果也显示了PSPA对心脏病理变化的保护作用。这些结果为应用PSPA作为功能性食品干预DOX诱导的心脏毒性提供了参考。