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Biotechnological Advances and Trends in Engineering Trichoderma reesei towards Cellulase Hyperproducer
Biotechnology and Bioprocess Engineering ( IF 3.2 ) Pub Date : 2021-07-19 , DOI: 10.1007/s12257-020-0243-y
Hao Fang 1, 2 , Chaofeng Li 1, 2 , Jiajia Zhao 1, 2 , Chen Zhao 1, 2
Affiliation  

Cellulase has the biggest contribution to the high production costs of lignocellulose bioconversion and the substantial decrease of its production cost is the key to the commercialization of lignocellulosic biorefineries. Trichoderma reesei has the most robust cellulase among the candidates, which therefore is widely used for cellulase production in industry. This is not because of the size of its cellulase gene pool but its prodigious cargo of cellulase productivity. Still, T. reesei cellulase falls far short of perfection in real-world applications, especially for the composition. This review summarized the biotechnological advances in engineering T. reesei for enhanced cellulase production. Meanwhile, we proposed innovative ideas of systematically optimizing cellulase composition at the transcriptional level and improving cellulase production at the regulation level. Efficient genome editing is essential to achieving that target. Thus, the developments of the tools of multiple gene manipulations were discussed in detail here. This review provides ideas and/or inspirations to the future researches on T. reesei cellulase.



中文翻译:

工程里氏木霉向纤维素酶超生产者的生物技术进展和趋势

纤维素酶对木质纤维素生物转化的高生产成本贡献最大,其生产成本的大幅降低是木质纤维素生物炼制商业化的关键。里氏木霉在候选者中具有最强的纤维素酶,因此被广泛用于工业上的纤维素酶生产。这不是因为其纤维素酶基因库的大小,而是因为其纤维素酶生产力的惊人货物。尽管如此,里氏木霉纤维素酶在实际应用中还远远不够完美,尤其是在组合物方面。本综述总结了工程里氏木霉的生物技术进展以提高纤维素酶的产量。同时,我们提出了在转录水平上系统优化纤维素酶组成和在调控水平上提高纤维素酶产量的创新思路。有效的基因组编辑对于实现该目标至关重要。因此,这里详细讨论了多基因操作工具的发展。该综述为里氏木霉纤维素酶的未来研究提供了思路和/或启发。

更新日期:2021-07-20
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