Herein we report a case of a 29-year-old man, suffering from a disease of unknown etiology and indicated a history of a bite by the unknown flying insect to both ear lobes in his anamnesis. The patient developed several nonspecific symptoms, such as fever, tension headache, and bilateral swelling of cervical lymph nodes (lymphadenitis). Moreover, he described perspiration, shivering, chills, epistaxis, several collapses, myalgia, and bone pains. Successive medical examinations of the patient confirmed several diagnoses. In addition, a blood sample was examined for the presence of tick-borne pathogens. PCR assay targeting gltA gene fragment of Rickettsia spp. brought a positive result. DNA of Rickettsia–like organism was confirmed by PCR and further sequencing. The rickettsial infection was successfully treated with doxycycline with a significant improvement of his health. This study brings a report of the molecular evidence of Rickettsia-like infection in a human patient from Slovakia, who suffered from several health problems accompanied by nonspecific febrile clinical symptoms. Although the patient did not report the tick bite before the development of clinical symptoms, he reported that he was bitten by an unknown flying insect. Thus, the possibility of rickettsial infection should be considered in similar nonspecific febrile illnesses when the bite of a flying insect is reported in personal anamnesis.

在此，我们报告了一个 29 岁男子的病例，他患有一种病因不明的疾病，并表明他的既往史中的两个耳垂都曾被不明飞虫叮咬过。患者出现了几种非特异性症状，如发热、紧张性头痛和双侧颈部淋巴结肿大（淋巴结炎）。此外，他还描述了出汗、发抖、发冷、鼻衄、几次虚脱、肌痛和骨痛。对患者的连续体检证实了多项诊断。此外，还检查了血液样本中是否存在蜱传病原体。靶向立克次体属gltA基因片段的PCR测定。带来了积极的结果。立克次体DNA样生物通过 PCR 和进一步测序得到证实。用强力霉素成功治疗立克次体感染，他的健康状况显着改善。这项研究报告了斯洛伐克一名患有多种健康问题并伴有非特异性发热临床症状的人类患者的类立克次体感染的分子证据。尽管患者在出现临床症状之前没有报告被蜱虫叮咬，但他报告说他被一种不知名的飞虫咬伤。因此，当在个人病历中报告飞虫叮咬时，在类似的非特异性发热性疾病中应考虑立克次体感染的可能性。