The discovery of potential, new cost-effective drug resources in the form of bioactive compounds from mushrooms is one way to control the resistant pathogens. In the present research, the fruiting bodies of five wild mushrooms were collected from Egypt and identified using internal transcribed spacer region (ITS) of the rRNA encoding gene and their phylogenetic relationships, antimicrobial activities, and biochemical and phenolic compounds were evaluated. The sequences revealed identity to Bjerkandera adusta, Cyclocybe cylindracea, Agrocybe aegerita, Chlorophyllum molybdites, and Lentinus squarrosulus in which Cyclocybe cylindracea and Agrocybe aegerita were closely related, while Chlorophyllum molybdites was far distant. Cyclocybe cylindracea and Agrocybe aegerita showed 100% similarity based on the sequenced ITS-rDNA fragment and dissimilar antimicrobial activities and chemical composition were detected. Bjerkandera adusta and Cyclocybe cylindracea showed strong antimicrobial activity against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Micrococcus luteus, Streptococcus pneumoniae, and Candida albicans. This activity could be attributed to the detected phenolic and related compounds’ contents. Our finding provides a quick and robust implement for mushroom identification that would facilitate mushroom domestication and characterization for human benefit.

中文翻译：

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一些野生埃及蘑菇的分子鉴定和抗菌活性：Bjerkandera adusta 作为生物活性抗菌酚类化合物的有希望的来源

从蘑菇中以生物活性化合物的形式发现潜在的、具有成本效益的新药物资源是控制耐药病原体的一种方法。在本研究中，从埃及采集了五种野生蘑菇的子实体，并使用 rRNA 编码基因的内部转录间隔区 (ITS) 对其进行了鉴定，并对它们的系统发育关系、抗菌活性以及生化和酚类化合物进行了评估。序列揭示了Bjerkandera adusta、Cyclocybe cylindracea、Agrocybe aegerita、Chlorophyllum molybdites 和Lentinus squaresulus，其中Cyclocybe cylindracea 和Agrocybe aegerita 密切相关，而叶绿素则相距很远。Cyclocybe cylindracea 和 Agrocybe aegerita 基于测序的 ITS-rDNA 片段表现出 100% 的相似性，并检测到不同的抗菌活性和化学成分。Bjerkandera adusta 和 Cyclocybe cylindracea 对大肠杆菌、铜绿假单胞菌、金黄色葡萄球菌、藤黄微球菌、肺炎链球菌和白色念珠菌显示出很强的抗菌活性。这种活性可归因于检测到的酚类和相关化合物的含量。我们的发现为蘑菇识别提供了一种快速而强大的工具，这将促进蘑菇的驯化和表征，以造福人类。金黄色葡萄球菌、藤黄微球菌、肺炎链球菌和白色念珠菌。这种活性可归因于检测到的酚类和相关化合物的含量。我们的发现为蘑菇识别提供了一种快速而强大的工具，这将促进蘑菇的驯化和表征，以造福人类。金黄色葡萄球菌、藤黄微球菌、肺炎链球菌和白色念珠菌。这种活性可归因于检测到的酚类和相关化合物的含量。我们的发现为蘑菇识别提供了一种快速而强大的工具，这将促进蘑菇的驯化和表征，以造福人类。