Biofunctionalization was investigated for polymers and metals considering their scarce integration ability. On the contrary few studies dealt with ceramic biofunctionalization because the bioactive and bioresorbable surfaces of ceramics are able to positively interact with biological environment. In this study the cell-response improvement on biofunctionalized wollastonite and diopside-based scaffolds was demonstrated. The ceramics were first obtained by heat treatment of a silicone embedding reactive oxide fillers and then biofunctionalized with adhesive peptides mapped on vitronectin. The most promising in vitro results, in terms of h-osteoblast proliferation and bone-related gene expression, were reached anchoring selectively a peptide stable toward proteolytic degradation induced by serum-enriched medium. In in vivo assays the anchoring of this protease-stable adhesive peptide was combined with self-assembling peptides, for increasing cell viability and angiogenesis. The results demonstrated external and internal cell colonization of biofunctionalized scaffolds with formation of new blood vessels (neoangiogenesis) and stimulation of ectopic mineralization.

考虑到它们稀缺的整合能力，研究了聚合物和金属的生物功能化。相反，很少有研究涉及陶瓷生物功能化，因为陶瓷的生物活性和生物可吸收表面能够与生物环境产生积极的相互作用。在这项研究中，证明了生物功能化硅灰石和透辉石基支架的细胞反应改善。陶瓷首先是通过对嵌入活性氧化物填料的有机硅进行热处理获得的，然后用映射在玻连蛋白上的粘合肽进行生物功能化。最有希望的体外实验结果，就成骨细胞增殖和骨相关基因表达而言，达到了选择性锚定对由富含血清的培养基诱导的蛋白水解降解稳定的肽。在体内试验中，这种蛋白酶稳定的粘附肽的锚定与自组装肽相结合，以增加细胞活力和血管生成。结果表明生物功能化支架的外部和内部细胞定植与新血管的形成（新血管生成）和异位矿化的刺激。