Peripheral blood mononuclear cells (PBMCs) have shown promise as a tissue sensitive to subtle and possibly systemic transcriptomic changes, and as such may be useful in identifying responses to weight loss interventions. The primary aim was to comprehensively evaluate the transcriptomic changes that may occur during weight loss and to determine if there is a consistent response across intervention types in human populations of all ages. Included studies were randomised control trials or cohort studies that administered an intervention primarily designed to decrease weight in any overweight or obese human population. A systematic search of the literature was conducted to obtain studies and gene expression databases were interrogated to locate corresponding transcriptomic datasets. Datasets were normalised using the ArrayAnalysis online tool and differential gene expression was determined using the limma package in R. Over-represented pathways were explored using the PathVisio software. Heatmaps and hierarchical clustering were utilised to visualise gene expression. Seven papers met the inclusion criteria, five of which had raw gene expression data available. Of these, three could be grouped into high responders (HR, ≥ 5% body weight loss) and low responders (LR). No genes were consistently differentially expressed between high and low responders across studies. Adolescents had the largest transcriptomic response to weight loss followed by adults who underwent bariatric surgery. Seven pathways were altered in two out of four studies following the intervention and the pathway ‘cytoplasmic ribosomal proteins’ (WikiPathways: WP477) was altered between HR and LR at baseline in the two datasets with both groups. Pathways related to ‘toll-like receptor signalling’ were altered in HR response to the weight loss intervention in two out of three datasets. Transcriptomic changes in PBMCs do occur in response to weight change. Transparent and standardised data reporting is needed to realise the potential of transcriptomics for investigating phenotypic features. PROSPERO: CRD42019106582

中文翻译：

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外周血单个核细胞的转录组学变化与体重减轻：系统文献综述和原始数据合成

外周血单核细胞 (PBMC) 已显示出作为对细微和可能的全身转录组变化敏感的组织的前景，因此可能有助于确定对减肥干预的反应。主要目的是全面评估减肥过程中可能发生的转录组变化，并确定所有年龄段人群的干预类型是否有一致的反应。纳入的研究是随机对照试验或队列研究，其实施的干预主要旨在减轻任何超重或肥胖人群的体重。对文献进行系统搜索以获得研究，并询问基因表达数据库以定位相应的转录组数据集。使用 ArrayAnalysis 在线工具对数据集进行标准化，并使用 R 中的 limma 包确定差异基因表达。使用 PathVisio 软件探索过度表达的途径。热图和层次聚类被用来可视化基因表达。七篇论文符合纳入标准，其中五篇具有可用的原始基因表达数据。其中，三个可分为高反应者（HR，体重减轻 ≥ 5%）和低反应者 (LR)。在整个研究中，高反应者和低反应者之间没有一致的基因差异表达。青少年对体重减轻的转录组反应最大，其次是接受减肥手术的成年人。干预后四项研究中有两项改变了七条通路，通路“细胞质核糖体蛋白”（WikiPathways：WP477) 在两组的两个数据集中基线 HR 和 LR 之间发生改变。在三个数据集中的两个数据集中，与“toll 样受体信号传导”相关的通路在 HR 对减肥干预的反应中发生了改变。PBMC 的转录组学变化确实会响应体重变化而发生。需要透明和标准化的数据报告来实现转录组学研究表型特征的潜力。普罗斯佩罗：CRD42019106582 需要透明和标准化的数据报告来实现转录组学研究表型特征的潜力。普罗斯佩罗：CRD42019106582 需要透明和标准化的数据报告来实现转录组学研究表型特征的潜力。普罗斯佩罗：CRD42019106582