Overusing nitrogen fertilizer not only pollute farmlands, but also make Nilaparvata lugens (Stål) less susceptible to pesticides. The current study investigated the relationship between nitrogen fertilizer application and buprofenzin resistance in N. lugens in rice by conducting a series of field efficacy tests and laboratory bioassays. The results showed that the efficacy of buprofezin decreased significantly with high level of nitrogen fertilizer application, compared to those with low or no nitrogen application in rice fields. In the laboratory, the resistance ratios of N. lugens to buprofezin, assayed at three concentrations of nitrogen (0, 1.0, 3.0 mmol/L), have developed to 14.05-, 18.40- and 25.81-fold after 20 generations of resistance selection, respectively. Meanwhile, the resistance ratios of N. lugens without buprofezin treated were 1.00-, 3.30- and 4.67-fold after 20 generations as the nitrogen concentrations increased. The enzymatic assays revealed that the activities of P450 and esterase increased significantly after resistance selection with buprofezin and nitrogen, especially on the esterase activity in N. lugens under the condition of nitrogen treatment. These results suggested that nitrogen fertilizer application contributed to the resistance development in N. lugens to buprofezin by enhancing the activity of esterase.

过度使用氮肥不仅会污染农田，还会使Nilaparvata lugens (Stål) 不易受到农药的影响。目前的研究通过进行一系列田间药效试验和实验室生物测定，研究了氮肥施用与水稻褐飞虱抗性之间的关系。结果表明，与稻田低施氮或不施氮肥相比，高施氮肥水平显着降低丁苯丙嗪的药效。在实验室中，褐飞虱的抗性比在三种氮浓度（0、1.0、3.0 mmol/L）下检测到的对丁苯丙酸的耐药性在经过 20 代抗性选择后分别发展到 14.05、18.40 和 25.81 倍。同时，随着氮浓度的增加，未经 buprofezin 处理的N. lugens的抗性比在 20 代后分别增加了 1.00、3.30 和 4.67 倍。酶学检测结果表明，通过buprofezin和氮进行抗性选择后，P450和酯酶的活性显着增加，特别是氮处理条件下褐飞虱的酯酶活性。这些结果表明，施氮肥有助于N. lugens的抗性发展 通过提高酯酶的活性来合成丁苯丙酸。