Objective An electronegative electroretinogram (ERG) can indicate important ocular or systemic disease. This study explored the prevalence of electronegative responses to dark-adapted stimuli in a largely healthy cohort. Methods and Analysis 211 participants recruited from the TwinsUK cohort underwent ERG testing incorporating international standard (International Society for Clinical Electrophysiology of Vision (ISCEV)) protocols and additional stimuli. Responses were recorded using conductive fibre electrodes, following pupil dilation and 20 min dark adaptation. Responses analysed were to the ISCEV standard and strong flashes (3.0 and 10 cd/m2 s), and to additional white flashes (0.67–67 cd/m2 s). A-wave and b-wave amplitudes were extracted; b:a ratios were calculated and proportions of eyes with ratios<1 were noted. Results Mean (SD) age was 62.4 (11.4) years (median, 64.3; range 23–86 years). 93% were female. Mean (SD) b:a ratios for right and left eyes, respectively, were 1.86 (0.33) and 1.81 (0.29) for the standard flash, and 1.62 (0.25) and 1.58 (0.23) for the stronger flash; average b:a ratio was lower for the stronger flash (p<0.0001). No waveforms were electronegative. For additional flashes, b:a ratio decreased with increasing flash strength. No electronegative waveforms were seen except in three eyes (0.7%) for the strongest flash; in some cases, drift in the waveform may have artefactually reduced the b:a ratio. Conclusion For standard dark-adapted stimuli, no participants had electronegative waveforms. The findings support the notion that electronegative waveforms (in response to standard flash strengths) are unusual, and should prompt further investigation. Data are available upon request.

中文翻译：

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健康成人队列中负电性视网膜电图的患病率

目的 负电性视网膜电图 (ERG) 可以提示重要的眼部或全身疾病。这项研究探讨了在大部分健康人群中对暗适应刺激的负电反应的发生率。方法与分析 从 TwinsUK 队列中招募的 211 名参与者接受了结合国际标准（国际临床视觉电生理学会 (ISCEV)）方案和额外刺激的 ERG 测试。在瞳孔扩张和 20 分钟暗适应后，使用导电纤维电极记录反应。分析了对 ISCEV 标准和强闪光（3.0 和 10 cd/m2 s）以及额外白色闪光（0.67-67 cd/m2 s）的响应。提取A波和B波振幅；计算b:a比率并记录比率<1的眼睛的比例。结果 平均 (SD) 年龄为 62.4 (11.4) 岁（中位数 64.3；范围 23-86 岁）。93%是女性。对于标准闪光，右眼和左眼的平均 (SD) b:a 比率分别为 1.86 (0.33) 和 1.81 (0.29)，对于更强闪光，分别为 1.62 (0.25) 和 1.58 (0.23)；闪光越强，平均 b:a 比率越低 (p<0.0001)。没有波形呈负电性。对于额外的闪光，b:a 比率随着闪光强度的增加而降低。除了三只眼睛 (0.7%) 的最强闪光外，没有看到负电波形；在某些情况下，波形漂移可能会人为地降低 b:a 比率。结论 对于标准暗适应刺激，没有参与者出现电负性波形。研究结果支持这样的观点，即负电性波形（响应标准闪光强度）是不寻常的，应该促使进一步研究。数据可根据要求提供。