Understanding bacterial adsorption and the evolution of biofilms on arsenopyrite with different surface structures is of great significance to clarifying the mechanism of microbe-mineral interfacial interactions and the production of acidic mine drainage impacting the environment. In this study, the attachment of Sulfobacillus thermosulfidooxidans cells and subsequent biofilm formation on arsenopyrite with different surface structures in the presence of dissolved As(III) was studied. Arsenopyrite slices with a specific surface were obtained by electrochemical corrosion at 0.26 V. The scanning electronic microscopy-energy dispersion spectra analyses indicated that the arsenopyrite surface deficient in sulfur and iron obtained by electrochemical treatment was not favorable for the initial adsorption of bacteria, and the addition of As(III) inhibited the adsorption of microbial cells. Epifluorescence microscopy showed that the number of cells attaching to the arsenopyrite surface increased with time; however, biofilm formation was delayed significantly when As(III) was added.

了解不同表面结构毒砂上的细菌吸附和生物膜的演化，对于阐明微生物-矿物界面相互作用的机制和酸性矿山排水对环境的影响具有重要意义。在本研究中，热硫化氧化硫杆菌的附着研究了在溶解的 As(III) 存在下具有不同表面结构的毒砂上的细胞和随后的生物膜形成。通过0.26 V的电化学腐蚀获得具有比表面的毒砂切片。扫描电镜-能量色散谱分析表明，通过电化学处理获得的缺乏硫和铁的毒砂表面不利于细菌的初始吸附， As(III)的加入抑制了微生物细胞的吸附。荧光显微镜显示，附着在毒砂表面的细胞数量随时间增加；然而，当添加 As(III) 时，生物膜的形成显着延迟。