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Tellurite Adsorption onto Bacterial Surfaces
Environmental Science & Technology ( IF 11.4 ) Pub Date : 2021-07-19 , DOI: 10.1021/acs.est.1c01001
Jennifer L Goff 1 , Yuwei Wang 2 , Maxim I Boyanov 3, 4 , Qiang Yu 5 , Kenneth M Kemner 4 , Jeremy B Fein 5 , Nathan Yee 1, 2
Affiliation  

Tellurium (Te) is an emerging contaminant and its chemical transformation in the environment is strongly influenced by microbial processes. In this study, we investigated the adsorption of tellurite [Te(IV), TeO32–] onto the common soil bacterium Bacillus subtilis. Thiol-blocking experiments were carried out to investigate the role of cell surface sulfhydryl sites in tellurite binding, and extended X-ray absorption fine structure (EXAFS) spectroscopy was performed to determine the chemical speciation of the adsorbed tellurite. The results indicate that tellurite reacts with sulfhydryl functional groups in the extracellular polymeric substances (EPS) produced by B. subtilis. Upon binding to sulfhydryl sites in the EPS, the Te changes from Te–O bonds to Te–S coordination. Further analysis of the surface-associated molecules shows that the EPS of B. subtilis contain proteins. Removal of the proteinaceous EPS dramatically decreases tellurite adsorption and the sulfhydryl surface site concentration. These findings indicate that sulfhydryl binding in EPS plays a key role in tellurite adsorption on bacterial surfaces.

中文翻译:

亚碲酸盐吸附到细菌表面

碲 (Te) 是一种新兴污染物,其在环境中的化学转化受到微生物过程的强烈影响。在这项研究中,我们研究了亚碲酸盐 [Te(IV), TeO 3 2- ] 对常见土壤细菌枯草芽孢杆菌的吸附。进行了硫醇阻断实验以研究细胞表面巯基位点在碲酸盐结合中的作用,并进行扩展 X 射线吸收精细结构 (EXAFS) 光谱以确定吸附的碲酸盐的化学形态。结果表明,亚碲酸盐与枯草芽孢杆菌产生的胞外聚合物(EPS)中的巯基官能团发生反应. 在与 EPS 中的巯基位点结合后,Te 从 Te-O 键变为 Te-S 配位。对表面相关分子的进一步分析表明,枯草芽孢杆菌的 EPS含有蛋白质。去除蛋白质EPS显着降低亚碲酸盐吸附和巯基表面位点浓度。这些发现表明 EPS 中的巯基结合在细菌表面的碲酸盐吸附中起关键作用。
更新日期:2021-08-03
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