The purpose of this in vitro study was to evaluate the effect of immediate dentin sealing (IDS) with and without chlorhexidine (CHX) pretreatment on the shear bond strength (SBS) of dual-cure adhesive resin cement. Mid-coronal dentin surfaces were obtained from 75 human molars. They were randomly allocated into five groups in accordance to type of IDS [etch&rinse/ER (Adper Single Bond2) and universal/U adhesive systems (Single Bond Universal)] and presence of CHX application (n = 15): Group ER; Group ER + CHX; Group U; Group U + CHX; and Group C (no IDS). Dual-cure adhesive resin cements were bonded with a cylinder-shaped Teflon mold. The SBS was measured using a universal test machine. Fracture type was evaluated with stereomicroscope. The resin/dentin interfaces were examined with an environmental scanning electron microscope. Data were statistically analyzed with two-way analysis of variance and Bonferroni tests (p < .05). Regarding the IDS treatment groups with/without CHX, there were no significant differences in SBS (p > .05). Group U + CHX showed significantly higher SBS than Group C (p < .05). Regarding the presence of CHX, no significant differences in SBS were found (p > .05). The prevalent failure mode was the mixed type for most of the groups. Group C exhibited an intact and regular hybrid layer with no resin tag, whereas longer and clear resin-tag formation was visible for Group U + CHX. CHX pretreatment improved the bond strength between adhesive resin cement and dentin when IDS treatment was performed with a universal adhesive system.

中文翻译：

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氯己定预处理即刻牙本质封闭对双固化胶粘剂剪切粘结强度的影响

这项体外研究的目的是评估使用和不使用氯己定 (CHX) 预处理的即刻牙本质封闭 (IDS) 对双固化粘合树脂水门汀的剪切粘合强度 (SBS) 的影响。从 75 颗人类磨牙获得中冠牙本质表面。根据 IDS [蚀刻&冲洗/ER (Adper Single Bond2) 和通用/U 粘合剂系统 (Single Bond Universal)] 的类型和 CHX 应用的存在 ( n = 15)：ER 组；ER + CHX 组；U组；U组+CHX；和 C 组（无 IDS）。双固化粘合树脂水门汀用圆柱形聚四氟乙烯模具粘合。使用万能试验机测量 SBS。用立体显微镜评估骨折类型。树脂/牙本质界面用环境扫描电子显微镜检查。数据采用方差分析和 Bonferroni 检验的双向分析 ( p  < .05)进行统计分析。对于有/没有 CHX 的 IDS 治疗组，SBS 没有显着差异 ( p  > .05)。U + CHX 组的 SBS 显着高于 C 组 ( p  < .05)。关于 CHX 的存在，未发现 SBS 存在显着差异（p > .05）。大多数组的普遍故障模式是混合类型。C 组表现出完整且规则的混合层，没有树脂标签，而 U + CHX 组则可见更长且清晰的树脂标签形成。当使用通用粘合剂系统进行 IDS 处理时，CHX 预处理提高了粘合剂树脂水门汀和牙本质之间的粘合强度。