The tetrazole fused pyrimidine system possesses a broad spectrum of biological activities. So, we have synthesized 5-(substitutedphenyl)-7-(1H- imidazol-4-yl)-4,7-dihydrotetrazolo[1,5-a]pyrimidine derivatives (5a-n) by reaction of chalcones with 5-aminotetrazole without catalyst in appropriate solvent. The structural elucidation of these compounds is based on MS, IR, 1H-NMR and 13C-NMR spectral data. The in vitro anti-microbial activity was investigated against Gram-positive and Gram-negative bacterial and fungal strains. It was found that the compounds 5a, 5b, 5c, 5d, 5e showed significant activities against tested organisms as compared to standard drugs (Ampicillin and Griseofulvin) while compounds 5a, 5c, 5i, and 5k showed good percentage of average inhibition in the dormant and active stage of tuberculosis.

中文翻译：

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一种简便、高效且无催化剂的咪唑、四唑和嘧啶组合部分作为潜在抗氧化剂的合成

四唑稠合嘧啶系统具有广泛的生物活性。因此，我们已经合成了5-（取代的苯基）-7-（1 ħ -咪唑-4-基）-4,7-二dihydrotetrazolo [1,5-一个]嘧啶衍生物（5A-N）通过与5-查耳酮的反应氨基四唑而不都是适宜催化剂ë溶剂。这些化合物的结构解析基于 MS、IR、1 H-NMR 和13 C-NMR 光谱数据。针对革兰氏阳性和革兰氏阴性细菌和真菌菌株研究了体外抗微生物活性。发现化合物5a , 5b , 5c , 5d，5E相比于标准的药物（氨苄西林和灰黄霉素），而化合物显示对所测试生物体显著活动图5a ，图5c ，图5I ，和5K显示在结核病的休眠和活动期平均抑制的良好百分比。