Blood cell analysis is a major pillar of biomedical research and healthcare. These analyses are performed in central laboratories. Rapid shipment from collection site to the central laboratories is currently needed because cells and biomarkers degrade rapidly. The dried blood spot from a fingerstick allows the preservation of cellular molecules for months but entire cells are never recovered. Here leucocyte elution is optimized from dried blood spots. Flow cytometry and mRNA expression profiling are used to analyze the recovered cells. 50–70% of the leucocytes that are dried on a polyester solid support via elution after shaking the support with buffer are recovered. While red blood cells lyse upon drying, it is found that the majority of leucocytes are preserved. Leucocytes have an altered structure that is improved by adding fixative in the elution buffer. Leucocytes are permeabilized, allowing an easy staining of all cellular compartments. Common immunophenotyping and mRNAs are preserved. The ability of a new biomarker (CD169) to discriminate between patients with and without Severe Acute Respiratory Syndrome induced by Coronavirus 2 (SARS-CoV-2) infections is also preserved. Leucocytes from blood can be dried, shipped, and/or stored for at least 1 month, then recovered for a wide variety of analyses, potentially facilitating biomedical applications worldwide.

中文翻译：

---

干血斑细胞分析：免疫学、血液学和传染病学的新机遇

血细胞分析是生物医学研究和医疗保健的主要支柱。这些分析在中心实验室进行。由于细胞和生物标志物会迅速降解，因此目前需要从收集地点快速运送到中央实验室。指尖上的干血斑可以将细胞分子保存数月，但永远无法恢复整个细胞。在这里，从干血斑中优化白细胞洗脱。流式细胞术和 mRNA 表达谱用于分析回收的细胞。50–70% 的白细胞被回收，这些白细胞在用缓冲液摇动支持物后通过洗脱在聚酯固体支持物上干燥。虽然红细胞在干燥时溶解，但发现大部分白细胞被保留下来。通过在洗脱缓冲液中添加固定剂可以改善白细胞的结构。白细胞被透化，可以轻松染色所有细胞区室。保留了常见的免疫表型和 mRNA。新的生物标志物 (CD169) 能够区分是否患有由冠状病毒 2 (SARS-CoV-2) 感染引起的严重急性呼吸系统综合症的患者。血液中的白细胞可以干燥、运输和/或储存至少 1 个月，然后回收用于各种分析，有可能促进全球生物医学应用。新的生物标志物 (CD169) 能够区分是否患有由冠状病毒 2 (SARS-CoV-2) 感染引起的严重急性呼吸系统综合症的患者。血液中的白细胞可以干燥、运输和/或储存至少 1 个月，然后回收用于各种分析，有可能促进全球生物医学应用。新的生物标志物 (CD169) 能够区分是否患有由冠状病毒 2 (SARS-CoV-2) 感染引起的严重急性呼吸系统综合症的患者。血液中的白细胞可以干燥、运输和/或储存至少 1 个月，然后回收用于各种分析，有可能促进全球生物医学应用。