Here we introduce zapalog-mediated endoplasmic reticulum trap (zapERtrap), which allows one to use light to precisely trigger forward trafficking of diverse integral membrane proteins from internal secretory organelles to the cell surface with single cell and subcellular spatial resolution. To demonstrate its utility, we use zapERtrap in neurons to dissect where synaptic proteins emerge at the cell surface when processed through central (cell body) or remote (dendrites) secretory pathways. We reveal rapid and direct long-range trafficking of centrally processed proteins deep into the dendritic arbor to synaptic sites. Select proteins were also trafficked to the plasma membrane of the axon initial segment, revealing a novel surface trafficking hotspot. Proteins locally processed through dendritic secretory networks were widely dispersed before surface insertion, challenging assumptions for precise trafficking at remote sites. These experiments provide new insights into compartmentalized secretory trafficking and showcase the tunability and spatiotemporal control of zapERtrap, which will have broad applications for regulating cell signaling and function.

中文翻译：

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zapERtrap：光调节内质网释放系统揭示了意想不到的神经元运输途径

在这里，我们介绍了zapalog介导的内质网陷阱（zapERtrap），它允许人们利用光以单细胞和亚细胞空间分辨率精确触发多种整合膜蛋白从内部分泌细胞器到细胞表面的正向运输。为了证明其实用性，我们在神经元中使用 zapERtrap 来剖析突触蛋白在通过中央（细胞体）或远程（树突）分泌途径进行处理时在细胞表面出现的位置。我们揭示了中央加工蛋白深入树突乔木到突触位点的快速和直接的远距离运输。选择的蛋白质也被运输到轴突起始段的质膜，揭示了一个新的表面运输热点。通过树突分泌网络本地加工的蛋白质在表面插入之前广泛分散，这对远程站点精确运输的假设提出了挑战。这些实验为区室化分泌运输提供了新的见解，并展示了 zapERtrap 的可调性和时空控制，这将在调节细胞信号传导和功能方面具有广泛的应用。