Nitrogen deficiency affects soybean growth and physiology, such as symbiosis with rhizobia; however, its effects on the bacterial composition of the soybean root microbiota remain unclear. A bacterial community analysis by 16S rRNA gene amplicon sequencing showed nitrogen deficiency-induced bacterial community shifts in soybean roots with the marked enrichment of Methylobacteriaceae. The abundance of Methylobacteriaceae was low in the roots of field-grown soybean without symptoms of nitrogen deficiency. Although Methylobacteriaceae isolated from soybean roots under nitrogen deficiency did not promote growth or nodulation when inoculated into soybean roots, these results indicate that the enrichment of Methylobacteriaceae in soybean roots is triggered by nitrogen-deficiency stress.

中文翻译：

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大豆根中氮缺乏引起的细菌群落变化。

缺氮影响大豆生长和生理，例如与根瘤菌共生；然而，其对大豆根系微生物群细菌组成的影响仍不清楚。通过 16S rRNA 基因扩增子测序进行的细菌群落分析显示，大豆根中氮缺乏引起的细菌群落发生变化，甲基杆菌科明显富集。没有缺氮症状的大田大豆根中甲基杆菌科的丰度较低。尽管从大豆根部分离出的甲基杆菌科在接种到大豆根部时没有促进生长或结瘤，但这些结果表明大豆根部甲基杆菌科的富集是由缺氮胁迫引发的。