Fast photochemical oxidation of proteins (FPOP) has demonstrated the ability to inform on the higher order structure of proteins. Recent technological advances have extended FPOP to live cells (IC-FPOP) using multiple cell lines and in vivo (IV-FPOP) using C. elegans. These innovations allow proteins to be studied in their native cellular environment. Hydroxyl radicals are generated via the photoloysis of hydrogen peroxide. Hydrogen peroxide is a signaling molecule that can induce changes to some proteins in the cell limiting the proteins that can be studied by IC-FPOP. Here, we evaluate the sulfate radical anion as a footprinting label in IC-FPOP with sodium persulfate as the precursor. Our findings show a 1.5-fold increase in the number of modified proteins compared to IC-FPOP using hydroxyl radicals at the same precursor concentration demonstrating the amenability of this radical with IC-FPOP.

中文翻译：

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评估硫酸根阴离子作为细胞内蛋白质快速光化学氧化的新试剂。

蛋白质的快速光化学氧化 (FPOP) 已证明能够了解蛋白质的高级结构。最近的技术进步已将 FPOP 扩展到使用多种细胞系的活细胞 (IC-FPOP) 和使用秀丽隐杆线虫的体内 (IV-FPOP)。这些创新允许在其天然细胞环境中研究蛋白质。羟基自由基是通过过氧化氢的光解作用产生的。过氧化氢是一种信号分子，可以诱导细胞中某些蛋白质发生变化，从而限制了 IC-FPOP 可以研究的蛋白质。在这里，我们评估硫酸根阴离子作为 IC-FPOP 中的足迹标签，以过硫酸钠为前体。我们的研究结果显示 1。