Intravital imaging is a powerful technology used to quantify and track dynamic changes in live cells and tissues within an intact environment. The ability to watch cell biology in real-time 'as it happens' has provided novel insight into tissue homeostasis, as well as disease initiation, progression and response to treatment. In this minireview, we highlight recent advances in the field of intravital microscopy, touching upon advances in awake versus anaesthesia-based approaches, as well as the integration of biosensors into intravital imaging. We also discuss current challenges that, in our opinion, need to be overcome to further advance the field of intravital imaging at the single-cell, subcellular and molecular resolution to reveal nuances of cell behaviour that can be targeted in complex disease settings.

中文翻译：

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使用活体成像量化和可视化体内细胞动力学的细微差别。

活体成像是一项强大的技术，用于量化和跟踪完整环境中活细胞和组织的动态变化。“在发生时”实时观察细胞生物学的能力为组织稳态以及疾病的发生、进展和对治疗的反应提供了新的见解。在这篇小综述中，我们重点介绍了活体显微镜领域的最新进展，涉及清醒与基于麻醉的方法的进展，以及生物传感器与活体成像的集成。我们还讨论了当前的挑战，我们认为需要克服这些挑战以进一步推进活体成像领域的单细胞、亚细胞和分子分辨率，以揭示可在复杂疾病环境中靶向的细胞行为的细微差别。