Wound healing and tissue regeneration are orchestrated by the cellular microenvironment, e.g. the extracellular matrix (ECM). Including ECM components in biomaterials is a promising approach for improving regenerative processes, e.g. wound healing in skin. This review addresses recent findings for enhanced epidermal-dermal regenerative processes on collagen (coll)/glycosaminoglycan (GAG)-based matrices containing sulfated GAG (sGAG) in simple and complex in vitro models. These matrices comprise 2D-coatings, electrospun nanofibrous scaffolds, and photo-crosslinked acrylated hyaluronan (HA-AC)/coll-based hydrogels. They demonstrated to regulate keratinocyte and fibroblast migration and growth, to stimulate melanogenesis in melanocytes from the outer root sheath (ORS) of hair follicles and to enhance the epithelial differentiation of human mesenchymal stem cells (hMSC). The matrices’ suitability for delivery of relevant growth factors, like heparin-binding epidermal growth factor like growth factor (HB-EGF), further highlights their potential as bioinspired, functional microenvironments for enhancing skin regeneration.

中文翻译：

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用于控制皮肤细胞反应的胶原蛋白/糖胺聚糖基质

伤口愈合和组织再生由细胞微环境协调，例如细胞外基质 (ECM)。在生物材料中加入 ECM 成分是改善再生过程的有前途的方法，例如皮肤伤口愈合。这篇综述讨论了在简单和复杂的含有硫酸化 GAG (sGAG) 的基于胶原蛋白 (coll)/糖胺聚糖 (GAG) 的基质上增强表皮-真皮再生过程的最新发现体外楷模。这些基质包括二维涂层、电纺纳米纤维支架和光交联丙烯酸化透明质酸 (HA-AC)/coll 基水凝胶。他们证明可以调节角质形成细胞和成纤维细胞的迁移和生长，刺激来自毛囊外根鞘 (ORS) 的黑色素细胞中的黑色素生成，并增强人间充质干细胞 (hMSC) 的上皮分化。该基质适用于递送相关生长因子，如肝素结合表皮生长因子样生长因子 (HB-EGF)，进一步突出了它们作为仿生功能性微环境促进皮肤再生的潜力。