Molecular imaging aims to depict the molecules in living patients. However, because this aim is still far beyond reach, patchworks of different solutions need to be used to tackle this overarching goal. From the vast toolbox of imaging techniques, we focus on those recent advances in optical microscopy that image molecules and cells at the submicron to centimeter scale. Mesoscopic imaging covers the "imaging gap" between techniques such as confocal microscopy and magnetic resonance imagingthat image entire live samples but with limited resolution. Microscopy focuses on the cellular level; mesoscopy visualizes the organization of molecules and cells into tissues and organs. The correlation between these techniques allows us to combine disciplines ranging from whole body imaging to basic research of model systems. We review current developments focused on improving microscopic and mesoscopic imaging technologies and on hardware and software that push the current sensitivity and resolution boundaries.

中文翻译：

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跨尺度最大化内容：将多模态显微镜和介观向分子成像移动。

分子成像旨在描绘活体患者的分子。然而，由于这个目标仍然遥不可及，需要使用不同解决方案的拼凑来解决这个总体目标。从庞大的成像技术工具箱中，我们专注于光学显微镜的最新进展，这些进展在亚微米到厘米尺度上对分子和细胞进行成像。细观成像涵盖了诸如共聚焦显微镜和磁共振成像之类的技术之间的“成像差距”，它们对整个活体样本进行成像，但分辨率有限。显微镜关注细胞水平；介观观察将分子和细胞组织成组织和器官。这些技术之间的相关性使我们能够结合从全身成像到模型系统基础研究的学科。