Selenium (Se) is an important trace element to maintain the body's dynamic balance. Lack of Se can cause inflammation. Studies have shown that inflammation often leads to disorders of the hypothalamic-pituitary-adrenal axis, but the mechanism by which Se deficiency causes inflammation of the porcine adrenal glands is still unclear. In order to study the effect of Se deficiency on the adrenal glands of pigs, we obtained Se-deficient pig adrenal glands through a low-Se diet. The results of mass spectrometry showed that the Se content in the Se-deficient group was only one-tenth of the control group. We detected the expression of the toll-like receptor 4 (TLR4) and downstream factors by qRT-PCR and Western blotting, and found that the lack of Se affected the TLR4/NF-κB pathway. It is known that miR-155-3p, miR-30d-R_1, and miR-146b have all been verified for targeting relationship with TLR4. We confirmed by qRT-PCR that miR-30d-R_1 decreased most significantly in the Se-deficient pig model. Then we tested 25 selenoproteins and some indicators of oxidative stress. It is confirmed that Se deficiency reduces the antioxidant capacity and induces oxidative stress in pig adrenal tissue. In short, a diet lacking Se induces oxidative stress in pig adrenal tissues and leads to inflammation through the miR-30d-R_1/TLR4 pathway. This study provides a reference for the prevention of adrenal inflammation in pigs from a nutritional point of view.

中文翻译：

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缺硒饮食通过 miR-30d-R_1 激活 TLR4/NF-κB 通路诱导猪肾上腺炎症反应。

硒（Se）是维持机体动态平衡的重要微量元素。缺乏硒会引起炎症。研究表明，炎症常导致下丘脑-垂体-肾上腺轴功能紊乱，但硒缺乏引起猪肾上腺炎症的机制尚不清楚。为了研究缺硒对猪肾上腺的影响，我们通过低硒日粮获得了缺硒猪肾上腺。质谱结果表明，缺硒组的硒含量仅为对照组的十分之一。我们通过qRT-PCR和Western印迹检测了toll样受体4（TLR4）和下游因子的表达，发现硒的缺乏影响了TLR4/NF-κB通路。已知 miR-155-3p、miR-30d-R_1、和 miR-146b 均已验证与 TLR4 的靶向关系。我们通过 qRT-PCR 证实 miR-30d-R_1 在缺硒猪模型中下降最为显着。然后我们测试了 25 种硒蛋白和一些氧化应激指标。已经证实，硒缺乏会降低猪肾上腺组织的抗氧化能力并诱导氧化应激。简而言之，缺乏硒的饮食会在猪肾上腺组织中诱导氧化应激，并通过 miR-30d-R_1/TLR4 通路导致炎症。本研究从营养学角度为猪肾上腺炎症的预防提供参考。已经证实，硒缺乏会降低猪肾上腺组织的抗氧化能力并诱导氧化应激。简而言之，缺乏硒的饮食会在猪肾上腺组织中诱导氧化应激，并通过 miR-30d-R_1/TLR4 通路导致炎症。本研究从营养学角度为猪肾上腺炎症的预防提供参考。已经证实，硒缺乏会降低猪肾上腺组织的抗氧化能力并诱导氧化应激。简而言之，缺乏硒的饮食会在猪肾上腺组织中诱导氧化应激，并通过 miR-30d-R_1/TLR4 通路导致炎症。本研究从营养学角度为猪肾上腺炎症的预防提供参考。