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Tumor Necrosis Factor-stimulated Gene-6 (TSG-6) Secreted by BMSCs Regulates Activated Astrocytes by Inhibiting NF-κB Signaling Pathway to Ameliorate Blood Brain Barrier Damage After Intracerebral Hemorrhage
Neurochemical Research ( IF 4.4 ) Pub Date : 2021-06-19 , DOI: 10.1007/s11064-021-03375-1
Bin Tang 1 , Min Song 2 , Xun Xie 2 , Dongsheng Le 3 , Qiulin Tu 3 , Xiang Wu 3 , Min Chen 3
Affiliation  

To investigate the influence of tumor necrosis factor-stimulated gene-6 (TSG-6) secreted by bone mesenchymal stem cells (BMSCs) on blood brain barrier (BBB) after intracerebral hemorrhage (ICH) and its related mechanisms. BMSCs and astrocytes were isolated and induced by TNF-α and LPS respectively. The effect of TSG-6 secreted by BMSCs on the proliferation and apoptosis of astrocytes and inflammatory response were assessed by CCK8, flow cytometry, and ELISA respectively. Then we studied the effects of TSG-6 secreted by BMSCs through the paracrine mechanism on the integrity of BBB after ICH via NF-κB signaling pathway in vitro and in vivo. We successfully isolated BMSCs and astrocytes. After LPS treatment of astrocytes, IL-1β, IL-6, and TNF-α showed an upward trend. TSG-6 secreted by TNF-α-activated BMSCs could antagonize the inflammatory response in activated astrocytes. Through the co-culture of astrocytes and BMSCs and the ICH animal model, we found that TSG-6 regulates activated astrocytes by inhibiting the NF-κB signaling pathway and ameliorates BBB damage. Furthermore, we found that TNF-α-activated BMSCs secreted exosomes containing TSG-6 and played an anti-inflammatory effect. TSG-6 secreted by BMSCs regulates activated astrocytes by inhibiting the NF-κB signaling pathway, thereby ameliorating BBB damage.



中文翻译:

骨髓间充质干细胞分泌的肿瘤坏死因子刺激基因 6 (TSG-6) 通过抑制 NF-κB 信号通路调节活化的星形胶质细胞以改善脑出血后血脑屏障损伤

探讨骨间充质干细胞(BMSCs)分泌的肿瘤坏死因子刺激基因6(TSG-6)对脑出血(ICH)后血脑屏障(BBB)的影响及其相关机制。分别用 TNF-α 和 LPS 分离和诱导 BMSCs 和星形胶质细胞。BMSCs分泌的TSG-6对星形胶质细胞增殖、凋亡和炎症反应的影响分别通过CCK8、流式细胞术和ELISA进行评估。然后我们在体外和体内通过NF-κB信号通路研究了BMSCs通过旁分泌机制分泌的TSG-6对ICH后BBB完整性的影响。我们成功分离了 BMSCs 和星形胶质细胞。LPS处理星形胶质细胞后,IL-1β、IL-6和TNF-α呈上升趋势。TNF-α 激活的 BMSCs 分泌的 TSG-6 可以拮抗激活的星形胶质细胞的炎症反应。通过星形胶质细胞和骨髓间充质干细胞的共培养以及 ICH 动物模型,我们发现 TSG-6 通过抑制 NF-κB 信号通路来调节活化的星形胶质细胞并改善 BBB 损伤。此外,我们发现TNF-α激活的BMSCs分泌含有TSG-6的外泌体并发挥抗炎作用。BMSCs 分泌的 TSG-6 通过抑制 NF-κB 信号通路调节活化的星形胶质细胞,从而改善 BBB 损伤。我们发现TNF-α激活的BMSCs分泌含有TSG-6的外泌体并发挥抗炎作用。BMSCs 分泌的 TSG-6 通过抑制 NF-κB 信号通路调节活化的星形胶质细胞,从而改善 BBB 损伤。我们发现TNF-α激活的BMSCs分泌含有TSG-6的外泌体并发挥抗炎作用。BMSCs 分泌的 TSG-6 通过抑制 NF-κB 信号通路调节活化的星形胶质细胞,从而改善 BBB 损伤。

更新日期:2021-07-24
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