We studied the mechanisms by which microRNA-126 regulates proliferation and migration of human umbilical vein endothelial cells (HUVEC) cultured in a medium with high glucose concentration and treated with chemokine CXCL8. Cell proliferation, apoptosis, and migration were analyzed by the CCK-8 assay, Annexin V-PI staining, and Transwell assay, respectively. The ratios of p-ERK/ERK, p-P38/P38, p-JNK/JNK were determined by ELISA. HUVEC cells cultured in the presence of high glucose concentration (30 mmol/ml) and treated with CXCL8 (50 ng/ml) demonstrated more intensive proliferation, migration, and p-ERK/ERK, p-P38/P38, and p-JNK/JNK ratios and significantly lower apoptosis rate than control cells (high glucose, no treatment) and cells treated with CXCL8 and transfected with microRNA-126-mimic. Thus, microRNA-126 regulates proliferation and migration of HUVEC cells cultured in the presence of high glucose concentrations and treated with CXCL8 through inhibition of MAPK signaling pathway.

中文翻译：

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MicroRNA-126 减弱趋化因子 CXCL8 对在高葡萄糖浓度培养基中培养的血管内皮细胞增殖、迁移、凋亡和 MAPK 依赖性信号活性的影响。

我们研究了 microRNA-126 调节在高葡萄糖浓度培养基中培养并用趋化因子 CXCL8 处理的人脐静脉内皮细胞 (HUVEC) 增殖和迁移的机制。分别通过 CCK-8 测定、Annexin V-PI 染色和 Transwell 测定分析细胞增殖、凋亡和迁移。通过ELISA确定p-ERK/ERK、p-P38/P38、p-JNK/JNK的比率。在高葡萄糖浓度 (30 mmol/ml) 存在下培养并用 CXCL8 (50 ng/ml) 处理的 HUVEC 细胞表现出更强烈的增殖、迁移和 p-ERK/ERK、p-P38/P38 和 p-JNK /JNK 比率和凋亡率显着低于对照细胞（高葡萄糖，未处理）和用 CXCL8 处理并用 microRNA-126-模拟物转染的细胞。因此，