MicroRNA (miR)-17-5p has been investigated in many diseases as a regulator of disease progression and is highly expressed in acute myeloid leukemia (AML). However, potential mechanisms underlying the function of miR-17-5p in AML need more elucidation. MiR-17-5p expression was augmented, while 25(OH)D3 and Beclin-1 levels were decreased in AML patients with the highest risk for disease progression. MiR-17-5p, 25(OH)D3 and Beclin-1 were determined to be clinically important in AML based on ROC curve analysis. Higher miR-17-5p expression as well as lower 25(OH)D3 and Beclin-1 expression were relevant with poor prognosis in AML. In addition, miR-17-5p was negatively correlated with and bound to BECN1. Vitamin D was found to diminish cell proliferation and enhance autophagy. Finally, through rescue assays, miR-17-5p facilitated the ability of cell proliferation, inhibited autophagy and apoptosis by modulating Beclin-1 in HL-60 cells following the treatment of 4 μM vitamin D. Vitamin D promoted autophagy in AML cells by modulating miR-17-5p and Beclin-1.

中文翻译：

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维生素 D 通过抑制 miR-17-5p 诱导的 Beclin-1 过表达来促进 AML 细胞的自噬。

MicroRNA (miR)-17-5p 已在许多疾病中作为疾病进展的调节因子进行了研究，并且在急性髓性白血病 (AML) 中高度表达。然而，miR-17-5p 在 AML 中功能的潜在机制需要更多的阐明。在疾病进展风险最高的 AML 患者中，MiR-17-5p 表达增加，而 25(OH)D3 和 Beclin-1 水平降低。基于 ROC 曲线分析，确定 MiR-17-5p、25(OH)D3 和 Beclin-1 在 AML 中具有临床意义。较高的 miR-17-5p 表达以及较低的 25(OH)D3 和 Beclin-1 表达与 AML 的不良预后相关。此外，miR-17-5p与BECN1呈负相关并结合。发现维生素 D 可减少细胞增殖并增强自噬。最后，通过救援化验，