Objective

β-Carotene has been widely used in the food and feed industry and has significant commercial value. This study aimed to increase the β-carotene production in engineered Yarrowia lipolytica by optimizing the host metabolic network. The DID2 gene, a subunit of the endosomal sorting complex required for transport (ESCRT), was integrated into a β-carotene producing strain.

Results

The β-carotene production was increased by 260%, and the biomass increased by 10% for engineered Y. lipolytica. Meanwhile, DID2 elevated the mRNA level and protein level of the genes in the β-carotene synthesis pathway, then increased precursors (FPP, Lycopene) utilization. DID2 also increased the mRNA level of the genes in the glucose pathway, pentose phosphate pathway, and tricarboxylic acid cycle and promoted glucose utilization and cofactors consumption.

Conclusion

The ESCRT protein complex subunit, DID2, improved β-carotene production in engineered Y. lipolytica and beneficial to glucose utilization and cofactors consumption. This study provided new finding of the DID2 gene’s function and it mostly could be used for many other natural product productions.

中文翻译：

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通过在工程解脂耶氏酵母中过表达 ESCRT 复合物的一个亚基 DID2 基因来增强 β-胡萝卜素的产生

客观的

β-胡萝卜素已广泛应用于食品和饲料工业，具有显着的商业价值。本研究旨在通过优化宿主代谢网络来增加工程解脂耶氏酵母中β-胡萝卜素的产量。的DID2基因，内体排序复杂所需的运输（ESCRT）的亚基，被整合到一个β胡萝卜素生产菌株。

结果

工程解脂耶氏酵母的β-胡萝卜素产量增加了260%，生物量增加了10% 。同时，DID2提高了β-胡萝卜素合成途径中基因的mRNA水平和蛋白质水平，进而提高了前体（FPP、番茄红素）的利用率。DID2还增加了葡萄糖途径、磷酸戊糖途径和三羧酸循环中基因的 mRNA 水平，并促进了葡萄糖的利用和辅助因子的消耗。

结论

ESCRT 蛋白复合亚基DID2改善了工程解脂耶氏酵母中β-胡萝卜素的产生，并有利于葡萄糖利用和辅因子消耗。这项研究提供了DID2基因功能的新发现，它主要可用于许多其他天然产物的生产。