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miR-33-3p Regulates PC12 Cell Proliferation and Differentiation In Vitro by Targeting Slc29a1
Neurochemical Research ( IF 4.4 ) Pub Date : 2021-06-21 , DOI: 10.1007/s11064-021-03377-z
Bo-Quan Shan 1, 2, 3 , Wen Li 1, 2, 3 , Hui He 1, 2, 3 , He-Yan Zhao 1, 2, 3 , Mei-Ling Tian 1, 2, 3 , Xiang Cheng 1, 2, 3 , Jian-Bing Qin 1, 2, 3, 4 , Guo-Hua Jin 1, 2, 3, 4
Affiliation  

MicroRNA-33-3p (miR-33-3p) has been widely investigated for its roles in lipid metabolism and mitochondrial function; however, there are few studies on miR-33-3p in the context of neurological diseases. In this study, we investigated the functional role of miR-33-3p in rat pheochromocytoma PC12 cells. A miR-33-3p mimic was transduced into PC12 cells, and its effects on proliferation, apoptosis, and differentiation were studied using the MTS assay, EdU labeling, flow cytometry, qRT-PCR, western blot, ELISA, and immunofluorescence. We found that miR-33-3p significantly suppressed PC12 cell proliferation, but had no effect on apoptosis. Furthermore, miR-33-3p promoted the differentiation of PC12 cells into Tuj1-positive and choline acetyltransferase-positive neuron-like cells. Mechanistically, miR-33-3p repressed the expression of Slc29a1 in PC12 cells. Importantly, knocking down Slc29a1 in PC12 cells inhibited proliferation and induced differentiation into neuron-like cells. In conclusion, this study showed that miR-33-3p regulated Slc29a1, which in turn controlled the proliferation and differentiation of PC12 cells. Thus, we hypothesize that the miR-33-3p/Slc29a1 axis could be a promising therapeutic target for recovering neurons and the cholinergic nervous system.



中文翻译:

miR-33-3p 通过靶向 Slc29a1 在体外调控 PC12 细胞增殖和分化

MicroRNA-33-3p (miR-33-3p) 因其在脂质代谢和线粒体功能中的作用而被广泛研究。然而,在神经系统疾病的背景下,关于 miR-33-3p 的研究很少。在这项研究中,我们研究了 miR-33-3p 在大鼠嗜铬细胞瘤 PC12 细胞中的功能作用。将 miR-33-3p 模拟物转导到 PC12 细胞中,并使用 MTS 测定、EdU 标记、流式细胞术、qRT-PCR、蛋白质印迹、ELISA 和免疫荧光研究其对增殖、凋亡和分化的影响。我们发现 miR-33-3p 显着抑制 PC12 细胞增殖,但对细胞凋亡没有影响。此外,miR-33-3p 促进 PC12 细胞分化为 Tuj1 阳性和胆碱乙酰转移酶阳性神经元样细胞。从机制上讲,miR-33-3p 抑制 Slc29a1 在 PC12 细胞中的表达。重要的是,敲低 PC12 细胞中的 Slc29a1 可抑制增殖并诱导分化为神经元样细胞。总之,本研究表明,miR-33-3p 调控 Slc29a1,进而控制 PC12 细胞的增殖和分化。因此,我们假设 miR-33-3p/Slc29a1 轴可能是恢复神经元和胆碱能神经系统的有希望的治疗靶点。

更新日期:2021-07-24
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