In this research, we formulated new chemotherapeutic copper nanoparticles (Cu NPs) containing Allium noeanum Reut. ex Regel leaf for treating human endometrial cancer. For investigating the antioxidant activitiy, the 2,2-diphenyl-1-picrylhydrazyl (DPPH) test was used. MTT test was used on normal (Human umbilical vein endothelial cells (HUVECs)) and human endometrial cancer (Ishikawa, HEC-1-A, HEC-1-B, and KLE) cell lines for comparing the anti-human endometrial cancer properties of Cu(NO3)2, A. noeanum leaf aqueous extract, and copper nanoparticles. Copper nanoparticles had high cell death and anti-human endometrial cancer effects against Ishikawa, HEC-1-A, HEC-1-B, and KLE cell lines. The IC50 of A. noeanum leaf aqueous extract and copper nanoparticles against HEC-1-B cell line were 548 and 331 µg/mL, respectively; against HEC-1-A cell line were 583 and 356 µg/mL, respectively; against KLE cell line were 609 and 411 µg/mL, respectively; and against Ishikawa cell line were 560 and 357 µg/mL, respectively. Among the above cell lines, the best result of anti-human endometrial cancer properties of copper nanoparticles was gained in the cell line of HEC-1-B. This study indicated excellent anti-human endometrial cancer potentials of copper nanoparticles containing A. noeanum in the in vitro condition.

中文翻译：

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使用葱叶提取物、抗氧化剂和体外细胞毒性的铜纳米粒子 (Cu-NPs) 的生物合成。

在这项研究中，我们配制了含有 Allium noeanum Reut 的新型化学治疗铜纳米粒子 (Cu NPs)。ex Regel 叶用于治疗人类子宫内膜癌。为了研究抗氧化活性，使用了 2,2-diphenyl-1-picrylhydrazyl (DPPH) 测试。MTT测试用于正常（人脐静脉内皮细胞（HUVECs））和人子宫内膜癌（Ishikawa，HEC-1-A，HEC-1-B和KLE）细胞系，以比较抗人子宫内膜癌的特性Cu(NO3)2、A. noeanum 叶水提取物和铜纳米颗粒。铜纳米粒子对 Ishikawa、HEC-1-A、HEC-1-B 和 KLE 细胞系具有高细胞死亡和抗人类子宫内膜癌作用。A. noeanum 叶水提物和铜纳米颗粒对 HEC-1-B 细胞系的 IC50 分别为 548 和 331 µg/mL；对 HEC-1-A 细胞系的抑制分别为 583 和 356 µg/mL；针对 KLE 细胞系的浓度分别为 609 和 411 µg/mL；和对 Ishikawa 细胞系的分别为 560 和 357 µg/mL。在上述细胞系中，铜纳米粒子抗人子宫内膜癌特性的最佳结果是在HEC-1-B细胞系中获得的。该研究表明，在体外条件下，含有 A. noeanum 的铜纳米颗粒具有出色的抗人类子宫内膜癌潜力。