Oxidative stress has been proven to play a critical role in the pathogenesis of neuronal injury. As a novel adipocytokine, omentin is produced by visceral adipose with insulin sensitizing effects and has been revealed to possess anti-inflammatory effects. However, the possible effect of omentin on oxidative stress remains unknown. The present study aimed to detect the potential protective effect of omentin against hydrogen peroxide (H2O2)-induced cytotoxicity of PC12 cells. The results showed that no cytotoxic effect was shown in PC12 cells co-cultured with omentin alone at a concentration of 50-1000 ng/mL. The CCK8 and TUNEL assays suggested that omentin could remarkably attenuate apoptosis induced by 100 μM H2O2. The PCR and western blotting showed that the expression levels of Bax was significantly inhibited by omentin via the upregulation of miR-128-3p at its 3'-UTR. Taken together, these results indicated that omentin protects PC12 cells against H2O2-induced apoptosis, and further studies need to be conducted before utilization in the clinic for the treatment of neurodegenerative diseases.

中文翻译：

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omentin 对氧化应激诱导的 PC12 细胞凋亡的细胞保护作用。

氧化应激已被证明在神经元损伤的发病机制中起关键作用。作为一种新型脂肪细胞因子，网膜素由内脏脂肪产生，具有胰岛素增敏作用，并已被证明具有抗炎作用。然而，网膜蛋白对氧化应激的可能影响仍然未知。本研究旨在检测网膜素对过氧化氢 (H2O2) 诱导的 PC12 细胞毒性的潜在保护作用。结果表明，在与 50-1000 ng/mL 浓度的网膜蛋白共培养的 PC12 细胞中没有显示出细胞毒性作用。CCK8 和 TUNEL 测定表明 omentin 可以显着减弱 100 μM H2O2 诱导的细胞凋亡。PCR 和蛋白质印迹表明，网膜蛋白通过上调 miR-128-3p 的 3'-UTR 显着抑制 Bax 的表达水平。总之，这些结果表明 omentin 保护 PC12 细胞免受 H2O2 诱导的细胞凋亡，在临床用于治疗神经退行性疾病之前需要进一步研究。