Periodontitis is a chronic, multifactorial, inflammatory disease characterized by the progressive destruction of the periodontal tissues. Guided tissue regeneration (GTR), involving the use of barrier membranes, is one of the most successful clinical procedures for periodontal therapy. Nevertheless, rapid degradation of the membranes and membrane-related infections are considered two of the major reasons for GTR clinical failure. Recently, integration of non-antibiotic, antimicrobial materials to the membranes has emerged as a novel strategy to face the bacterial infection challenge, without increasing bacterial resistance. In this sense, bismuth subsalicylate (BSS) is a non-antibiotic, metal-based antimicrobial agent effective against different bacterial strains, that has been long safely used in medical treatments. Thus, the aim of the present work was to fabricate fibrillar, non-rapidly bioresorbable, antibacterial GTR membranes composed of polycaprolactone (PCL), gelatin (Gel), and BSS as the antibacterial agent. PCL-G-BSS membranes with three different BSS concentrations (2 wt./v%, 4 wt./v%, and 6 wt./v%) were developed by electrospinning and their morphology, composition, water wettability, mechanical properties, Bi release and degradation rate were characterized. The Cytotoxicity of the membranes was studied in vitro using human osteoblasts (hFOB) and gingival fibroblasts (HGF-1), and their antibacterial activity was tested against Aggregatibacter actinomycetemcomitans, Escherichia coli, Porphyromonas gingivalis and Staphylococcus aureus. The membranes obtained exhibited adequate mechanical properties for clinical application, and appropriate degradation rates for allowing periodontal defects regeneration. The hFOB and HGF-1 cells displayed adequate viability when in contact with the lixiviated products from the membranes, and, in general, displayed antibacterial activity against the four bacteria strains tested. Thus, the PCL-G-BSS membranes showed to be appropriate as potential barrier membranes for periodontal GTR treatments.

牙周炎是一种慢性、多因素的炎症性疾病，其特征是牙周组织进行性破坏。涉及使用屏障膜的引导组织再生 (GTR) 是牙周治疗最成功的临床程序之一。然而，膜的快速降解和膜相关感染被认为是 GTR 临床失败的两个主要原因。最近，将非抗生素、抗菌材料整合到膜上已成为一种新的策略来应对细菌感染挑战，而不会增加细菌耐药性。从这个意义上说，碱式水杨酸铋 (BSS) 是一种非抗生素、金属基抗菌剂，可有效对抗不同的细菌菌株，长期以来一直安全地用于医学治疗。因此，本工作的目的是制造由聚己内酯 (PCL)、明胶 (Gel) 和 BSS 作为抗菌剂组成的纤维状、非快速生物可吸收的抗菌 GTR 膜。通过静电纺丝技术开发了三种不同 BSS 浓度（2 wt./v%、4 wt./v% 和 6 wt./v%）的 PCL-G-BSS 膜及其形貌、组成、水润湿性、机械性能、表征了Bi的释放和降解速率。研究了膜的细胞毒性 对力学性能、Bi释放和降解速率进行了表征。研究了膜的细胞毒性 对力学性能、Bi释放和降解速率进行了表征。研究了膜的细胞毒性在体外使用人成骨细胞 (hFOB) 和牙龈成纤维细胞 (HGF-1)，并测试了它们对伴随放线菌、大肠杆菌、牙龈卟啉单胞菌和金黄色葡萄球菌的抗菌活性。获得的膜表现出足够的临床应用机械性能，以及允许牙周缺损再生的适当降解率。hFOB 和 HGF-1 细胞在与来自膜的浸提产物接触时显示出足够的活力，并且通常对所测试的四种细菌菌株显示出抗菌活性。因此，PCL-G-BSS 膜显示出适合作为牙周 GTR 治疗的潜在屏障膜。