A high-performance thin-layer chromatographic (HPTLC) method has developed for the selective detection of a diuretic drug, triamterene (TRIAM), in pure form, tablets and human plasma. The method was based on chromatographic separation of TRIAM using HPTLC plates, precoated with silica gel, and a mobile phase consisted of ethyl acetate: dimethylformamide: ammonia (7.0: 2.7: 0.3, by volume). The native fluorescence signal of TRIAM was detected at 440 nm and used to quantify TRIAM using the proposed method, improving the method sensitivity to ~250-folds in comparison to that reported in previous HPTLC studies. The developed method enabled the detection of TRIAM in pure drug and biological samples (human plasma) within linear concentrations ranged from 0.8 to 60 ng/band or 1.0 to 60 ng/band for pure drug and plasma samples, respectively. Furthermore, the method was validated according to the official guidelines to permit its applicability in quality control and clinical laboratories.

中文翻译：

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血浆中氨苯蝶啶超灵敏检测的 HPTLC 方法

一种高效薄层色谱 (HPTLC) 方法已开发用于选择性检测纯形式​​的利尿剂氨苯蝶啶 (TRIAM)、片剂和人血浆。该方法基于使用 HPTLC 板对 TRIAM 进行色谱分离，预涂有硅胶，流动相由乙酸乙酯：二甲基甲酰胺：氨水（体积比 7.0:2.7:0.3）组成。在 440 nm 处检测到 TRIAM 的天然荧光信号，并使用所提出的方法对 TRIAM 进行量化，与之前的 HPTLC 研究中报告的相比，方法灵敏度提高了约 250 倍。开发的方法能够检测纯药物和生物样品（人血浆）中的 TRIAM，其线性浓度范围分别为 0.8 至 60 ng/band 或 1.0 至 60 ng/band 的纯药物和血浆样品。此外，