In addition to nucleic acids, a variety of other biomolecules have also been found on the plasma membrane. Although researchers have realized that RNA has the ability to bind to membrane vesicles in vitro, little is known about whether and how RNA connects to the plasma membrane of the cell. The combination of high-throughput sequencing and in situ labeling methods provides an innovative approach for large-scale identification of subcellular RNAs. Here, we applied the recently published method APEX-seq and identified 75 RNAs related to the plasma membrane, in which lncRNA PMAR72 (plasma membrane-associated RNA AL121772.1) has a considerable affinity with sphingomyelin (SM) and localizes within distinct membrane foci. Our findings will provide some new evidence to elaborate the relationship between RNA and the plasma membrane of mammalian cells.

除核酸外，质膜上还发现了多种其他生物分子。尽管研究人员已经意识到 RNA 在体外具有与膜囊泡结合的能力，但对于 RNA 是否以及如何连接到细胞的质膜上却知之甚少。高通量测序和原位标记方法的结合为大规模鉴定亚细胞 RNA 提供了一种创新方法。在这里，我们应用最近发表的方法 APEX-seq 并确定了 75 个与质膜相关的 RNA，其中 lncRNA PMAR72（质膜相关 RNA AL121772.1）与鞘磷脂 (SM) 具有相当大的亲和力，并定位于不同的膜病灶内。我们的研究结果将为阐明RNA与哺乳动物细胞质膜之间的关系提供一些新的证据。